A Novel Preservation Solution Containing Quercetin and Sucrose for Porcine Kidney Transplantation.
Journal
Transplantation direct
ISSN: 2373-8731
Titre abrégé: Transplant Direct
Pays: United States
ID NLM: 101651609
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
18
03
2020
revised:
26
08
2020
accepted:
13
09
2020
entrez:
18
11
2020
pubmed:
19
11
2020
medline:
19
11
2020
Statut:
epublish
Résumé
In organ transplantation, the University of Wisconsin (UW) solution has been the gold standard for organ preservation. Quercetin (Que) has numerous antioxidant and anti-inflammatory activities, and sucrose (Suc) may be effective for cold storage (CS). This study aimed to investigate the in vitro protective effect of Que and Suc on cold injury to the kidney and to determine whether Que + Suc could improve ischemia-reperfusion injury during CS and hypothermic oxygenated perfusion (HOPE) in autologous transplantation models. BHK-21 cells were stored at 4°C for 3 days in UW solution for CS/machine perfusion (CS/MP-UW) with Que (33.1 μM, 3.3 μM, 0.33 μM) and Suc (0.1 M). In a porcine model of renal autologous transplantation, left kidney grafts were preserved under 3 conditions: group 1, CS preservation for 24 hours; group 2, CS preservation for 22 hours and HOPE with CS/MP-UW solution for 2 hours; and group 3, identical preservation as group 2, with Que and Suc added to the solution. Animals were euthanized on day 7 after autologous transplantation. After 3 days of CS preservation, the CS/MP-UW solution with Que (33.1 μM, 3.3 μM) and Suc showed significant cell protection against cold injury. In the porcine model of renal autologous transplantation, the last blood Cre level and the blood lipid hydroperoxide on posttransplantation day 2 were significantly different between group 1 and group 3. Moreover, the total endothelial, glomerular, tubular, interstitial (EGTI) histology score in the kidney tissue was also significantly different. Regarding the change in renal resistance in HOPE, the decrease observed in group 3 was significantly larger than that in group 2. Our results suggest that the addition of Que and Suc to a UW solution can improve kidney preservation and could potentially enhance the outcome of kidney transplantation.
Identifiants
pubmed: 33204822
doi: 10.1097/TXD.0000000000001077
pmc: PMC7665254
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e624Informations de copyright
Copyright © 2020 The Author(s). Transplantation Direct. Published by Wolters Kluwer Health, Inc.
Déclaration de conflit d'intérêts
The authors declare no funding or conflicts of interest.
Références
Ann Surg. 2013 Sep;258(3):409-21
pubmed: 24022434
Kidney Int. 2004 Jul;66(1):77-90
pubmed: 15200415
Transplant Proc. 2015 Jan-Feb;47(1):19-22
pubmed: 25645760
J Pharmacol Exp Ther. 2002 Jul;302(1):66-72
pubmed: 12065701
Liver Int. 2015 Jan;35(1):156-63
pubmed: 24351095
Eur J Pharmacol. 2008 May 13;585(2-3):325-37
pubmed: 18417116
Transplantation. 2000 Jan 27;69(2):249-58
pubmed: 10670635
Ann N Y Acad Sci. 1959 Feb 6;72(12):396-404
pubmed: 13627925
Free Radic Res. 2002 May;36(5):575-81
pubmed: 12150545
Prog Transplant. 2012 Jun;22(2):175-82
pubmed: 22878075
Acta Biochim Pol. 2001;48(1):183-9
pubmed: 11440168
J Surg Res. 2020 Jan;245:410-419
pubmed: 31437648
Phytother Res. 2008 Aug;22(8):1057-63
pubmed: 18570213
Cell Biochem Funct. 2002 Dec;20(4):291-6
pubmed: 12415562
Liver Transpl Surg. 1997 Jan;3(1):39-45
pubmed: 9377757
Oxid Med Cell Longev. 2009 Nov-Dec;2(5):259-69
pubmed: 20716913
J Invest Surg. 1990;3(1):65-75
pubmed: 2282350
Ann Transplant. 2020 Jan 14;25:e919920
pubmed: 31932575
Transplantation. 2019 May;103(5):e128-e136
pubmed: 30747849
Exp Ther Med. 2012 Feb;3(2):249-254
pubmed: 22969877
Am J Clin Nutr. 2005 Jan;81(1 Suppl):230S-242S
pubmed: 15640486
Transfus Med Hemother. 2011;38(2):125-142
pubmed: 21566713
Liver Transpl. 2017 Sep;23(9):1216-1225
pubmed: 28590542
Nephrol Dial Transplant. 2006 Feb;21(2):330-6
pubmed: 16221709
Am J Transl Res. 2017 Feb 15;9(2):429-441
pubmed: 28337272
Ann Surg. 2016 Nov;264(5):815-822
pubmed: 27584571
Nihon Yakurigaku Zasshi. 2015 Sep;146(3):140-3
pubmed: 26354013
Ann Surg. 2017 Feb;265(2):388-396
pubmed: 28059967
Kidney Int. 2003 Feb;63(2):554-63
pubmed: 12631120
Transpl Int. 2018 Jun;31(6):658-669
pubmed: 29493843
Free Radic Biol Med. 1993 Aug;15(2):227-32
pubmed: 8375697
Nihon Hinyokika Gakkai Zasshi. 1994 Jun;85(6):925-31
pubmed: 8065074
Peptides. 2003 Sep;24(9):1381-6
pubmed: 14706553
Transplantation. 2000 Nov 27;70(10):1424-31
pubmed: 11118084
Environ Toxicol Pharmacol. 2010 Mar;29(2):158-66
pubmed: 21787598
Kidney Int. 2006 Mar;69(5):884-93
pubmed: 16407886
Annu Rev Med. 1995;46:235-47
pubmed: 7598460
Transplant Proc. 2014 May;46(4):1095-8
pubmed: 24815137