PRMT1 inhibition induces differentiation of colon cancer cells.
Animals
Antineoplastic Agents
/ pharmacology
Apoptosis
Biomarkers, Tumor
/ genetics
Cell Differentiation
Cell Proliferation
Colonic Neoplasms
/ drug therapy
Gene Expression Regulation, Neoplastic
/ drug effects
Humans
Mice
Protein-Arginine N-Methyltransferases
/ antagonists & inhibitors
Repressor Proteins
/ antagonists & inhibitors
Tumor Cells, Cultured
Xenograft Model Antitumor Assays
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
18 11 2020
18 11 2020
Historique:
received:
14
05
2020
accepted:
04
11
2020
entrez:
19
11
2020
pubmed:
20
11
2020
medline:
2
4
2021
Statut:
epublish
Résumé
Differentiation therapy has been recently revisited as a prospective approach in cancer therapy by targeting the aberrant growth, and repairing the differentiation and cell death programs of cancer cells. However, differentiation therapy of solid tumors is a challenging issue and progress in this field is limited. We performed High Throughput Screening (HTS) using a novel dual multiplex assay to discover compounds, which induce differentiation of human colon cancer cells. Here we show that the protein arginine methyl transferase (PRMT) type 1 inhibitor, MS023, is a potent inducer of colon cancer cell differentiation with a large therapeutic window. Differentiation changes in the highly aggressive human colon cancer cell line (HT-29) were proved by proteomic and genomic approaches. Growth of HT-29 xenograft in nude mice was significantly delayed upon MS023 treatment and immunohistochemistry of tumor indicated differentiation changes. These findings may lead to development of clinically effective anti-cancer drugs based on the mechanism of cancer cell differentiation.
Identifiants
pubmed: 33208761
doi: 10.1038/s41598-020-77028-8
pii: 10.1038/s41598-020-77028-8
pmc: PMC7676271
doi:
Substances chimiques
Antineoplastic Agents
0
Biomarkers, Tumor
0
Repressor Proteins
0
PRMT1 protein, human
EC 2.1.1.319
Protein-Arginine N-Methyltransferases
EC 2.1.1.319
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
20030Références
Nat Protoc. 2014 Sep;9(9):2100-22
pubmed: 25101824
Nat Med. 2015 Dec;21(12):1473-80
pubmed: 26569382
Oncotarget. 2016 Oct 11;7(41):67532-67550
pubmed: 27556302
J Proteome Res. 2019 Mar 1;18(3):1441-1445
pubmed: 30761899
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Exp Cell Res. 2012 Aug 1;318(13):1577-85
pubmed: 22487097
Acta Neuropathol. 2017 Apr;133(4):629-644
pubmed: 28124097
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
J Dig Dis. 2014 Apr;15(4):159-65
pubmed: 24373518
Stem Cell Res. 2017 Aug;23:158-162
pubmed: 28756340
World J Biol Chem. 2014 May 26;5(2):115-29
pubmed: 24921003
Nat Med. 1998 Sep;4(9):1046-52
pubmed: 9734398
Arch Biochem Biophys. 1981 Sep;210(2):581-91
pubmed: 7305346
Bioinformatics. 2018 Oct 15;34(20):3600
pubmed: 29788404
Oncogene. 2020 Jan;39(5):987-1003
pubmed: 31591478
Cell. 2011 Mar 4;144(5):646-74
pubmed: 21376230
J Biol Chem. 2005 Sep 23;280(38):32890-6
pubmed: 16051612
FEBS Lett. 2011 Jul 7;585(13):2024-31
pubmed: 21074527
Clin Colorectal Cancer. 2017 Dec;16(4):247-251
pubmed: 28363756
J Pathol. 2017 Jun;242(2):178-192
pubmed: 28299802
ACS Chem Biol. 2016 Mar 18;11(3):772-781
pubmed: 26598975
Cancer Microenviron. 2015 Apr;8(1):15-21
pubmed: 25326055
Cancer Discov. 2016 May;6(5):492-500
pubmed: 26976114
Genome Biol. 2014;15(12):550
pubmed: 25516281
J Appl Physiol (1985). 2010 Aug;109(2):598-605
pubmed: 20203073
Cancer Cell. 2019 Jul 8;36(1):100-114.e25
pubmed: 31257072
Curr Colorectal Cancer Rep. 2011 Jun;7(2):121-127
pubmed: 21980310
Nat Biotechnol. 2008 Dec;26(12):1367-72
pubmed: 19029910
J Exp Med. 2002 Nov 18;196(10):1373-80
pubmed: 12438428
J Biol Chem. 1987 Jan 25;262(3):1092-7
pubmed: 3805014
Dev Biol. 2007 Sep 15;309(2):298-305
pubmed: 17706959
Nat Rev Drug Discov. 2010 Mar;9(3):203-14
pubmed: 20168317
Genes Dev. 2002 Jun 15;16(12):1488-97
pubmed: 12080087
J Biol Chem. 2014 Sep 5;289(36):25306-16
pubmed: 25037223
J Biol Chem. 2000 Mar 17;275(11):7723-30
pubmed: 10713084
Nat Rev Drug Discov. 2014 Aug;13(8):588-602
pubmed: 25033736
Mol Pharm. 2014 Dec 1;11(12):4395-404
pubmed: 25313982
Cancer Cell. 2015 Sep 14;28(3):343-56
pubmed: 26321221
J Biol Chem. 2002 Jul 12;277(28):24855-8
pubmed: 12023272
Ann Oncol. 2011 Mar;22(3):500-502
pubmed: 21343384
Br J Cancer. 2008 Dec 16;99(12):2094-9
pubmed: 19078953
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450
pubmed: 30395289
Cell Death Differ. 2011 Jul;18(7):1231-3
pubmed: 21212795
Br J Cancer. 1999 Nov;81(6):934-41
pubmed: 10576648
Drug Discov Today. 2013 Nov;18(21-22):1067-73
pubmed: 23850704
J Surg Res. 2016 May 1;202(1):225-34
pubmed: 27083970
Oncotarget. 2017 May 23;8(21):35326-35338
pubmed: 28186961
Nature. 2016 Jan 7;529(7584):97-100
pubmed: 26700806
Oncotarget. 2012 May;3(5):559-67
pubmed: 22643847
Epigenetics. 2008 Jan-Feb;3(1):28-37
pubmed: 18326939
Expert Opin Biol Ther. 2017 Jun;17(6):709-721
pubmed: 28375039
Molecules. 2015 Mar 02;20(3):3898-941
pubmed: 25738536
Nat Rev Cancer. 2018 Feb;18(2):117-127
pubmed: 29192213
Oncotarget. 2017 Jan 31;8(5):8854-8866
pubmed: 27750219
Cells Tissues Organs. 2007;185(1-3):61-5
pubmed: 17587809
CA Cancer J Clin. 2018 Nov;68(6):394-424
pubmed: 30207593
Lancet Oncol. 2015 Oct;16(13):1295-305
pubmed: 26384238
Mol Med Rep. 2015 Mar;11(3):1566-72
pubmed: 25384676
Exp Cell Res. 2011 Nov 15;317(19):2702-10
pubmed: 21978911
Curr Pathobiol Rep. 2016;4:135-145
pubmed: 27547510
Drug Discov Today. 2013 Mar;18(5-6):240-9
pubmed: 23073387
Cancer Res. 2018 Oct 15;78(20):5833-5847
pubmed: 30154152
J Cell Physiol. 1994 Oct;161(1):111-23
pubmed: 7929596
Mod Pathol. 2007 Dec;20(12):1286-97
pubmed: 17906616
Anticancer Res. 2009 Apr;29(4):1361-6
pubmed: 19414388
Anticancer Res. 2008 Nov-Dec;28(6A):3815-26
pubmed: 19189669