Levels of the oxidative stress biomarker malondialdehyde in tears of patients with central serous chorioretinopathy relate to disease activity.
Adult
Aminoacridines
/ metabolism
Biomarkers
/ metabolism
Case-Control Studies
Central Serous Chorioretinopathy
/ complications
Female
Humans
Male
Malondialdehyde
/ metabolism
Middle Aged
Multivariate Analysis
Oxidative Stress
Retinal Detachment
/ complications
Tears
/ metabolism
Thiobarbiturates
/ metabolism
Tomography, Optical Coherence
Journal
Molecular vision
ISSN: 1090-0535
Titre abrégé: Mol Vis
Pays: United States
ID NLM: 9605351
Informations de publication
Date de publication:
2020
2020
Historique:
received:
06
01
2020
accepted:
13
10
2020
entrez:
19
11
2020
pubmed:
20
11
2020
medline:
27
8
2021
Statut:
epublish
Résumé
Central serous chorioretinopathy (CSCR) has been associated with oxidative stress-related risk factors. The objective of this study was to optimize an analytical method for evaluating the oxidative stress biomarker malondialdehyde (MDA) in human tears and determine its level in the tears of patients with CSCR. In this pilot study, tear samples were obtained from 34 healthy donors and 31 treatment-naïve CSCR male patients (eight with acute CSCR and 23 with chronic CSCR). Two analytical methods based on high-performance liquid chromatography followed by fluorescence detection were evaluated, with either 2-thiobarbituric derivative (TBA) or 2-aminoacridone (2-AA). Activity of CSCR was defined by the serous retinal detachment (SRD) height, which was measured by two independent observers on spectral-domain optical coherence tomography. The 2-AA method showed higher sensitivity and precision compared to the TBA method. When the 2-AA method was applied to tears from healthy donors, the levels of MDA were statistically significantly higher in men compared to women (mean ± standard deviation, SD: 9,914 nM ± 6,126 versus 4,635 nM ± 1,173, p = 0.006). No difference was found in tear MDA levels between male patients with CSCR and age-matched control men (p = 0.17). However, MDA levels were statistically significantly higher in acute compared to chronic CSCR cases (mean ± SD: 12,295 nM ± 8,495 versus 6,790 ± 3,969 nM, p = 0.03). Additionally, there was a correlation between MDA levels and RPE leakage, quantified by the height of the serous retinal detachment (p = 0.02, r = 0.40). Levels of MDA in tears, measured with an optimized analytical method, correlate with RPE leakage in CSCR.
Substances chimiques
Aminoacridines
0
Biomarkers
0
Thiobarbiturates
0
2-aminoacridone
27918-14-5
Malondialdehyde
4Y8F71G49Q
thiobarbituric acid
M1YZW5SS7C
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
722-730Informations de copyright
Copyright © 2020 Molecular Vision.
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