Utility of nested polymerase chain reaction for fungus in detecting clinically suspected patients of invasive fungal infections and its clinical correlation and comparison with fungal culture.
Febrile neutropenia
invasive fungal infection
polymerase chain reaction
Journal
Journal of family medicine and primary care
ISSN: 2249-4863
Titre abrégé: J Family Med Prim Care
Pays: India
ID NLM: 101610082
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
05
05
2020
revised:
11
06
2020
accepted:
22
07
2020
entrez:
19
11
2020
pubmed:
20
11
2020
medline:
20
11
2020
Statut:
epublish
Résumé
The aim and objective of this study is to detect invasive fungal infections (IFIs) early and with more sensitivity by the nested polymerase chain reaction (PCR) for fungus as compared to fungal culture in clinically suspected patients and also explore its correlation in reference to age, duration of symptoms, immunocompromised status, and other risk factors predisposing to IFIs. In this cross-sectional study, 50 suspected patients admitted in medical acute care unit/intensive care unit (ACU/ICU) of Sir Sunderlal Hospital, Banaras Hindu University, Varanasi, India, comprised the study. All cases were selected based on the predefined inclusion and exclusion criteria. A detailed history, clinical examination, and all required investigations were done in all suspected patients. Blood samples were taken for nested-PCR for fungus and culture. Nested PCR was performed on extracted DNA form samples collected from all participants under the study. Our study comprised of 50 suspected immunocompromised patients of IFIs. Among the participants under the study, the most common risk factor was diabetes mellitus (28% cases). Nearly two-thirds (60%) of the cases were 50 years or more. Around 70% of the cases had a history of illness more than 2 weeks. Nested PCR for fungus came out to be positive in 21/50 patients (42%); however, fungal culture was positive in none. Among the admitted patient in ACU/ICU, 75% were neutropenic. IFIs are more common in immunocompromised individuals, patients with comorbidities, long history of symptoms, and elderly population. Nested PCR for fungus has a high sensitivity (as compared to the fungal culture), and also they are rapid in giving the results. Thus, nested PCR for fungus can be used in a cost-effective manner for the early and reliable diagnosis of clinically suspected IFIs.
Identifiants
pubmed: 33209834
doi: 10.4103/jfmpc.jfmpc_775_20
pii: JFMPC-9-4992
pmc: PMC7652180
doi:
Types de publication
Journal Article
Langues
eng
Pagination
4992-4997Informations de copyright
Copyright: © 2020 Journal of Family Medicine and Primary Care.
Déclaration de conflit d'intérêts
There are no conflicts of interest.
Références
Med Mycol. 2011 Apr;49 Suppl 1:S7-S12
pubmed: 20718606
Am J Respir Crit Care Med. 2004 Sep 15;170(6):621-5
pubmed: 15229094
J Infect Dev Ctries. 2011 Feb 01;5(1):75-8
pubmed: 21330746
J Heart Lung Transplant. 2011 Apr;30(4):361-74
pubmed: 21419994
J Clin Microbiol. 2002 Jul;40(7):2483-9
pubmed: 12089267
Clin Infect Dis. 2008 Jun 15;46(12):1813-21
pubmed: 18462102
Front Microbiol. 2020 Jan 14;10:2903
pubmed: 31993022
Med Mycol. 2002 Aug;40(4):333-57
pubmed: 12230214
Indian J Med Res. 2014 Feb;139(2):195-204
pubmed: 24718393
Int J Infect Dis. 2009 Jan;13(1):97-102
pubmed: 18672389
J Antimicrob Chemother. 2010 May;65(5):1042-51
pubmed: 20202987
Infection. 2013 Jun;41(3):645-53
pubmed: 23463186
Haematologica. 2006 Aug;91(8):1068-75
pubmed: 16885047
Support Care Cancer. 2006 May;14(5):469-74
pubmed: 16435098