Search for Viral Infections in Cerebrospinal Fluid From Patients With Autoimmune Encephalitis.
NGS
anti-NMDAR
autoimmune encephalitis
metagenomics
virus
Journal
Open forum infectious diseases
ISSN: 2328-8957
Titre abrégé: Open Forum Infect Dis
Pays: United States
ID NLM: 101637045
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
07
05
2020
accepted:
30
09
2020
entrez:
19
11
2020
pubmed:
20
11
2020
medline:
20
11
2020
Statut:
epublish
Résumé
It has been reported that virus-mediated brain tissue damage can lead to autoimmune encephalitis (AE) characterized by the presence of antibodies against neuronal surface antigens. In the study, we investigate the presence of viruses in cerebrospinal fluid (CSF) from patients with AE using reverse transcription polymerase chain reaction (RT-PCR)/PCR and shotgun metagenomics. CSF samples collected from 200 patients with encephalitis were tested for the presence of antibodies against antiglutamate receptor (NMDAR), contactin-associated protein 2 (CASPR2), glutamate receptors (type AMPA1/2), leucine-rich glioma-inactivated protein 1 (LGI1), dipeptidyl aminopeptidase-like protein 6 (DPPX), and GABA B receptor, and those found positive were further analyzed with real-time RT-PCR/PCR for common viral neuroinfections and shotgun DNA- and RNA-based metagenomics. Autoantibodies against neuronal cells were detected in CSF from 8 individuals (4% of all encephalitis patients): 7 (3.5%) had anti-NMDAR and 1 (0.5%) had anti-GABA B. RT-PCR/PCR identified human herpes virus type 1 (HSV-1; 300 copies/mL) and the representative of We detected the presence of HSV, TTV, and
Sections du résumé
BACKGROUND
BACKGROUND
It has been reported that virus-mediated brain tissue damage can lead to autoimmune encephalitis (AE) characterized by the presence of antibodies against neuronal surface antigens. In the study, we investigate the presence of viruses in cerebrospinal fluid (CSF) from patients with AE using reverse transcription polymerase chain reaction (RT-PCR)/PCR and shotgun metagenomics.
METHODS
METHODS
CSF samples collected from 200 patients with encephalitis were tested for the presence of antibodies against antiglutamate receptor (NMDAR), contactin-associated protein 2 (CASPR2), glutamate receptors (type AMPA1/2), leucine-rich glioma-inactivated protein 1 (LGI1), dipeptidyl aminopeptidase-like protein 6 (DPPX), and GABA B receptor, and those found positive were further analyzed with real-time RT-PCR/PCR for common viral neuroinfections and shotgun DNA- and RNA-based metagenomics.
RESULTS
RESULTS
Autoantibodies against neuronal cells were detected in CSF from 8 individuals (4% of all encephalitis patients): 7 (3.5%) had anti-NMDAR and 1 (0.5%) had anti-GABA B. RT-PCR/PCR identified human herpes virus type 1 (HSV-1; 300 copies/mL) and the representative of
CONCLUSIONS
CONCLUSIONS
We detected the presence of HSV, TTV, and
Identifiants
pubmed: 33209955
doi: 10.1093/ofid/ofaa468
pii: ofaa468
pmc: PMC7643957
doi:
Types de publication
Journal Article
Langues
eng
Pagination
ofaa468Informations de copyright
© The Author(s) 2020. Published by Oxford University Press on behalf of Infectious Diseases Society of America.
Références
Arch Immunol Ther Exp (Warsz). 2015 Feb;63(1):79-84
pubmed: 25376263
Neurol Neuroimmunol Neuroinflamm. 2018 Apr 05;5(4):e458
pubmed: 29629396
Neurol Neuroimmunol Neuroinflamm. 2020 Jan 3;7(2):
pubmed: 31900318
Neurology. 2015 Dec 15;85(24):2176-7
pubmed: 26567268
PLoS One. 2017 Jun 1;12(6):e0178481
pubmed: 28570620
Ann Neurol. 2012 Dec;72(6):902-11
pubmed: 23280840
Brain Behav. 2019 Dec;9(12):e01454
pubmed: 31650706
Exp Dermatol. 2008 May;17(5):446-54
pubmed: 18400063
J Clin Neurol. 2016 Jan;12(1):1-13
pubmed: 26754777
BMC Neurol. 2013 Oct 20;13:150
pubmed: 24139084
Neurology. 2015 Nov 17;85(20):1736-43
pubmed: 26491084
Pediatrics. 2020 Jun;145(6):
pubmed: 32358069
J Epilepsy Res. 2016 Dec 31;6(2):45-50
pubmed: 28101474
Curr Opin Neurol. 2014 Jun;27(3):361-8
pubmed: 24792345
BMC Neurol. 2019 Nov 6;19(1):273
pubmed: 31694559
Clin Infect Dis. 2006 Dec 15;43(12):1565-77
pubmed: 17109290
Brain. 2015 Jan;138(Pt 1):94-109
pubmed: 25392198
PLoS One. 2013 Apr 22;8(4):e61217
pubmed: 23630581
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
J Clin Invest. 1989 Oct;84(4):1174-80
pubmed: 2551924
Clin Immunol. 2019 Oct;207:97-99
pubmed: 31454696
BMC Neurol. 2017 Nov 6;17(1):195
pubmed: 29110638
Ann Neurol. 2018 Apr;83(4):863-869
pubmed: 29572931
Brain Behav Immun. 2020 Jul;87:179-181
pubmed: 32454137
Lancet Neurol. 2018 Sep;17(9):760-772
pubmed: 30049614
Viruses. 2019 Aug 19;11(8):
pubmed: 31430946
J Med Case Rep. 2019 Sep 24;13(1):302
pubmed: 31547860
Med Dosw Mikrobiol. 2012;64(2):139-49
pubmed: 23072059
Genes (Basel). 2019 Aug 29;10(9):
pubmed: 31470675
Neurol Neuroimmunol Neuroinflamm. 2018 Jun 11;5(4):e471
pubmed: 29904645
J Neurol. 2018 Oct;265(10):2190-2200
pubmed: 30003358
Med Dosw Mikrobiol. 2010;62(3):245-53
pubmed: 21114017
Clin Infect Dis. 2013 Oct;57(8):1114-28
pubmed: 23861361
J Virol. 2001 Apr;75(7):3077-88
pubmed: 11238834
Ther Adv Neurol Disord. 2017 Aug 16;11:1756285617722347
pubmed: 29399043
Adv Exp Med Biol. 2016 Jul 13;:
pubmed: 27405447
Arq Neuropsiquiatr. 2018 Jan;76(1):41-49
pubmed: 29364393
Seizure. 2020 Oct;81:18-20
pubmed: 32688169
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
Epilepsia Open. 2017 Jul 19;2(3):355-359
pubmed: 29588966
Front Immunol. 2017 Apr 21;8:442
pubmed: 28484451
Front Neurol. 2018 Oct 10;9:841
pubmed: 30364136
Lancet Neurol. 2016 Apr;15(4):391-404
pubmed: 26906964
Ann Neurol. 2014 Jul;76(1):108-19
pubmed: 24916964
Virology. 1999 Jul 5;259(2):428-36
pubmed: 10388666
Clin Immunol. 2005 Aug;116(2):124-34
pubmed: 15894513
Front Neurol. 2019 Jul 25;10:804
pubmed: 31404257
Med Dosw Mikrobiol. 2008;60(3):259-65
pubmed: 19143180
Eur Neurol. 2007;57(4):238-40
pubmed: 17389802
Clin Infect Dis. 2012 Apr;54(7):899-904
pubmed: 22281844
Medicine (Baltimore). 2017 Dec;96(49):e9177
pubmed: 29245365
Genome Res. 2011 Jun;21(6):936-9
pubmed: 20980556
Bioinformatics. 2009 Aug 15;25(16):2078-9
pubmed: 19505943
Pediatr Infect Dis J. 2016 Mar;35(3):347-9
pubmed: 26658379
Arch Pathol Lab Med. 2017 Jun;141(6):776-786
pubmed: 28169558
Sci Rep. 2019 Feb 20;9(1):2360
pubmed: 30787417
J Neurovirol. 2016 Feb;22(1):33-7
pubmed: 26139017
Neurol Neuroimmunol Neuroinflamm. 2018 Jan 09;6(2):e537
pubmed: 30697587
Eur Neurol Rev. 2013 Summer;8(1):31-37
pubmed: 27330568
Virol Sin. 2017 Dec;32(6):541-544
pubmed: 29047018
Lancet Infect Dis. 2010 Dec;10(12):835-44
pubmed: 20952256