In vitro mechanical vibration down-regulates pro-inflammatory and pro-fibrotic signaling in human vocal fold fibroblasts.
Actins
/ metabolism
Cells, Cultured
Cytokines
/ pharmacology
Down-Regulation
Extracellular Matrix
/ drug effects
Fibroblasts
/ cytology
Gene Expression Regulation
/ drug effects
Humans
Hyaluronic Acid
/ metabolism
Interleukin-11
/ metabolism
Interleukin-1beta
/ pharmacology
Models, Biological
Signal Transduction
/ drug effects
Stress, Physiological
Transforming Growth Factor beta1
/ pharmacology
Vibration
Vocal Cords
/ cytology
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
23
06
2020
accepted:
22
10
2020
entrez:
19
11
2020
pubmed:
20
11
2020
medline:
7
1
2021
Statut:
epublish
Résumé
Voice rest following phonotrauma or phonosurgery has a considerable clinical impact, but clinical recommendations are inconsistent due to inconclusive data. As biopsies of the vocal folds (VF) for molecular biology studies in humans are unethical, we established a new in vitro model to explore the effects of vibration on human vocal fold fibroblasts (hVFF) in an inflammatory and normal state, which is based on previously published models. By using a phonomimetic bioreactor we were able to apply predefined vibrational stress patterns on hVFF cultured under inflammatory or normal conditions. Inflammatory and pro-fibrotic stimuli were induced by interleukin (IL)1β and transforming growth factor (TGF)β1, respectively. Mechanical stimulation was applied four hours daily, over a period of 72 hours. Outcome measurements comprised assessment of extracellular matrix (ECM)-related components, angiogenic factors, and inflammatory and fibrogenic markers on gene expression and protein levels. Under inflammatory conditions, the inflammatory cytokine IL11, as well as the myofibroblast marker alpha smooth muscle actin (α-SMA) were significantly reduced when additional vibration was applied. The desirable anti-fibrotic ECM component hyaluronic acid was increased following cytokine treatment, but was not diminished following vibration. Our experiments revealed the effect of vibrational stress on hVFF in an inflammatory state. Elevated levels of certain pro-inflammatory/pro-fibrotic factors could be mitigated by additional vibrational excitation in an in vitro setting. These findings corroborate clinical studies which recommend early voice activation following an acute event.
Identifiants
pubmed: 33211714
doi: 10.1371/journal.pone.0241901
pii: PONE-D-20-19358
pmc: PMC7676657
doi:
Substances chimiques
ACTA2 protein, human
0
Actins
0
Cytokines
0
IL11 protein, human
0
Interleukin-11
0
Interleukin-1beta
0
Transforming Growth Factor beta1
0
Hyaluronic Acid
9004-61-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0241901Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Ann Otol Rhinol Laryngol. 2003 Dec;112(12):1021-5
pubmed: 14703104
Ann Otol Rhinol Laryngol. 2008 Feb;117(2):145-52
pubmed: 18357839
Biomatter. 2013 Jan-Mar;3(1):
pubmed: 23507923
Am J Physiol Cell Physiol. 2011 Aug;301(2):C272-9
pubmed: 21613612
Laryngoscope. 2015 Jun;125(6):E203-9
pubmed: 25545625
Laryngoscope. 2018 May;128(5):1170-1175
pubmed: 29105866
Logoped Phoniatr Vocol. 2018 Dec;43(4):143-154
pubmed: 30183437
Ann Transl Med. 2019 Feb;7(4):69
pubmed: 30963064
J Proteomics. 2017 Feb 23;155:11-21
pubmed: 28099887
J Voice. 2006 Mar;20(1):110-20
pubmed: 15964741
J Voice. 2015 Mar;29(2):133-9
pubmed: 25499520
Ann Otol Rhinol Laryngol. 2008 Mar;117(3):227-38
pubmed: 18444484
Sci Transl Med. 2019 Sep 25;11(511):
pubmed: 31554736
Laryngoscope. 2017 Jun;127(6):E193-E200
pubmed: 27774594
Laryngoscope. 2016 Mar;126(3):E110-7
pubmed: 26344050
Eur Arch Otorhinolaryngol. 2018 May;275(5):1211-1218
pubmed: 29520499
J Voice. 2017 Sep;31(5):520-527
pubmed: 28359643
PLoS One. 2010 Aug 16;5(8):e12196
pubmed: 20808940
Ann Otol Rhinol Laryngol. 2006 Dec;115(12):921-9
pubmed: 17214268
Genome Biol. 2002 Jun 18;3(7):RESEARCH0034
pubmed: 12184808
Curr Opin Otolaryngol Head Neck Surg. 2017 Dec;25(6):459-463
pubmed: 28857840
Laryngoscope. 2020 Jul;130(7):1750-1755
pubmed: 31498467
Lab Invest. 2016 Jul;96(7):807-16
pubmed: 27111284
J Voice. 2005 Jun;19(2):283-9
pubmed: 15907442
Laryngoscope. 2014 Dec;124(12):2770-8
pubmed: 25073715
Tissue Eng Part C Methods. 2016 Sep;22(9):823-38
pubmed: 27537192
Clin Exp Otorhinolaryngol. 2016 Mar;9(1):56-61
pubmed: 26976028
J Acoust Soc Am. 2013 Mar;133(3):1667-76
pubmed: 23464036
Nature. 2017 Dec 7;552(7683):110-115
pubmed: 29160304
Laryngoscope. 2010 Sep;120(9):1826-31
pubmed: 20717945
Laryngoscope. 2013 Mar;123(3):738-45
pubmed: 23444190
BMC Genomics. 2019 Apr 23;20(1):308
pubmed: 31014251
PLoS One. 2012;7(2):e30965
pubmed: 22359557
Auris Nasus Larynx. 2018 Aug;45(4):661-666
pubmed: 28844607
Clin Exp Immunol. 1999 Jan;115(1):176-82
pubmed: 9933439
Biotechniques. 2004 Jul;37(1):112-4, 116, 118-9
pubmed: 15283208
J Pathol. 2009 Mar;217(4):552-62
pubmed: 19097069
Cells Tissues Organs. 2017;204(2):93-104
pubmed: 28647731
J Arthroplasty. 2017 Aug;32(8):2604-2611
pubmed: 28285897
Ann Otol Rhinol Laryngol. 2000 Jul;109(7):658-66
pubmed: 10903048
Eur Arch Otorhinolaryngol. 2019 Feb;276(2):483-487
pubmed: 30631900
PLoS One. 2019 Mar 14;14(3):e0213788
pubmed: 30870529
J Voice. 2012 Nov;26(6):814.e1-13
pubmed: 23177745
Laryngoscope. 2020 Jun 22;:
pubmed: 32569447
J Voice. 2017 Jan;31(1):97-103
pubmed: 27492336
J Voice. 2015 Jan;29(1):13-21
pubmed: 25439508
Trends Biochem Sci. 2001 Oct;26(10):597-604
pubmed: 11590012
J Biomech. 2004 Oct;37(10):1521-9
pubmed: 15336927
Biomaterials. 2010 Mar;31(9):2542-8
pubmed: 20036421
Otolaryngol Head Neck Surg. 2008 Jan;138(1):62-8
pubmed: 18164995
Wound Repair Regen. 2008 Sep-Oct;16(5):585-601
pubmed: 19128254
J Voice. 2002 Sep;16(3):310-6
pubmed: 12395983
J Voice. 2017 Jan;31(1):129.e1-129.e7
pubmed: 26776951
Laryngoscope. 2010 Nov;120(11):2247-57
pubmed: 20830759
J Acoust Soc Am. 2007 Jan;121(1):469-78
pubmed: 17297801
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Clin Rehabil. 2015 Sep;29(9):844-54
pubmed: 25452634
Wound Repair Regen. 2016 Mar;24(2):215-22
pubmed: 26704519
Curr Opin Otolaryngol Head Neck Surg. 2019 Jun;27(3):185-190
pubmed: 30893134
Int Wound J. 2015 Jun;12(3):313-6
pubmed: 23742140
Front Biosci (Landmark Ed). 2012 Jun 01;17:2667-74
pubmed: 22652804
J Voice. 2010 Jul;24(4):379-87
pubmed: 19660903
Bone. 2009 Dec;45(6):1125-32
pubmed: 19665600
Cytokine. 2013 Jan;61(1):228-36
pubmed: 23123198
Mol Cell Proteomics. 2019 Aug;18(8):1511-1525
pubmed: 31123107
J Tissue Eng Regen Med. 2013 Mar;7(3):213-25
pubmed: 22095782
Wound Repair Regen. 2010 Jan-Feb;18(1):89-97
pubmed: 20002898
J Voice. 2007 Nov;21(6):651-60
pubmed: 16905293
Laryngoscope. 2018 Jul;128(7):E258-E264
pubmed: 29392734
Mol Ther Nucleic Acids. 2019 Jun 7;16:616-625
pubmed: 31100613
Exp Mol Med. 2020 Jan;52(1):130-151
pubmed: 31959867