FTIR Spectroscopy to Reveal Lipid and Protein Changes Induced on Sperm by Capacitation: Bases for an Improvement of Sample Selection in ART.
FTIR
infertility
lipids
sperm
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
17 Nov 2020
17 Nov 2020
Historique:
received:
13
10
2020
revised:
05
11
2020
accepted:
12
11
2020
entrez:
20
11
2020
pubmed:
21
11
2020
medline:
4
3
2021
Statut:
epublish
Résumé
Although being a crucial step for Assisted Reproduction Technologies (ART) success, to date sperm selection is based only on morphology, motility and concentration characteristics. Considering the many possible alterations, there is a great need for analytical approaches allowing more effective sperm selections. The use of Fourier Transform Infrared (FTIR) may represent an interesting possibility, being able to reveal many macromolecular changes in a single measurement in a nondestructive way. As a proof of concept, in this observational study, we used a FTIR approach to reveal features related to sperm quality and chemical changes promoted by in vitro capacitation. We found indication that α-helix content is increased in capacitated sperm, while high percentages of the β-structures seem to correlate to poor-quality spermatozoa. The most interesting observation was related to the lipid composition, when measured as CH
Identifiants
pubmed: 33212829
pii: ijms21228659
doi: 10.3390/ijms21228659
pmc: PMC7698301
pii:
doi:
Substances chimiques
Membrane Lipids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : IRCCS Burlo Garofolo/Italian ministery of health
ID : RC18/16; 5mille15D1.
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