Inhibitory effect of flavonoid xanthomicrol on triple-negative breast tumor via regulation of cancer-associated microRNAs.
angiogenesis
apoptosis
breast cancer
flavonoid
microRNA
xanthomicrol
Journal
Phytotherapy research : PTR
ISSN: 1099-1573
Titre abrégé: Phytother Res
Pays: England
ID NLM: 8904486
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
revised:
12
09
2020
received:
01
05
2020
accepted:
17
10
2020
pubmed:
21
11
2020
medline:
1
5
2021
entrez:
20
11
2020
Statut:
ppublish
Résumé
Breast cancer is the leading cause of cancer death in women worldwide. Due to the side effects of current chemo-reagents on healthy tissues, it is essential to search for alternative compounds with less toxicity and better efficacy. In the present study, we have investigated the anticancer effects of flavonoid xanthomicrol on the mice breast cancer model using MTT assay, cell cycle and Annexin/PI analysis, colony formation assay, H&E staining, immunohistochemistry, and miRNA analysis. Our results demonstrated that xanthomicrol decreased the cell viability and clonogenic capability, induced G1-arrest and apoptosis in the breast cancer cells in vitro, and caused a significant reduction in the volume and weight of mice tumors in vivo. In addition, xanthomicrol reduced the expression of TNFα, VEGF, MMP9, and Ki67, while upregulating the expression of apoptotic markers such as Bax, caspase3, and caspase9. Finally, the expression of miR21, miR27, and miR125, known as oncomirs, decreased significantly after xanthomicrol administration, while the expression of miR29 and miR34, functioning as tumor suppressors, increased significantly (p < .001). Our data demonstrated that xanthomicrol can induce apoptosis and suppress angiogenesis in breast cancer cells due to its inhibitory effect on oncomirs and its stimulatory effect on tumor suppressor miRNAs.
Substances chimiques
Flavones
0
Flavonoids
0
MicroRNAs
0
xanthomicrol
3IN82Y8CAA
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1967-1982Subventions
Organisme : Iran National Science Foundation
ID : 96009946
Informations de copyright
© 2020 John Wiley & Sons Ltd.
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