Membrane Tension Gates ERK-Mediated Regulation of Pluripotent Cell Fate.

Animals Cell Differentiation Embryonic Stem Cells Endocytosis Mice Mouse Embryonic Stem Cells Signal Transduction
Beta-catenin Cell fate choice Cell surface mechanics ERK Embryonic stem cells Endocytosis Membrane tension mechanical signalling pluripotency

Journal

Cell stem cell
ISSN: 1875-9777
Titre abrégé: Cell Stem Cell
Pays: United States
ID NLM: 101311472

Informations de publication

Date de publication:
04 02 2021
Historique:
received: 03 12 2019
revised: 18 09 2020
accepted: 28 10 2020
pubmed: 21 11 2020
medline: 20 5 2021
entrez: 20 11 2020
Statut: ppublish

Résumé

Cell fate transitions are frequently accompanied by changes in cell shape and mechanics. However, how cellular mechanics affects the instructive signaling pathways controlling cell fate is poorly understood. To probe the interplay between shape, mechanics, and fate, we use mouse embryonic stem cells (ESCs), which change shape as they undergo early differentiation. We find that shape change is regulated by a β-catenin-mediated decrease in RhoA activity and subsequent decrease in the plasma membrane tension. Strikingly, preventing a decrease in membrane tension results in early differentiation defects in ESCs and gastruloids. Decreased membrane tension facilitates the endocytosis of FGF signaling components, which activate ERK signaling and direct the exit from the ESC state. Increasing Rab5a-facilitated endocytosis rescues defective early differentiation. Thus, we show that a mechanically triggered increase in endocytosis regulates early differentiation. Our findings are of fundamental importance for understanding how cell mechanics regulates biochemical signaling and therefore cell fate.

Identifiants

DOI: 10.1016/j.stem.2020.10.018 PMID: 33217323 PMC: PMC7875115
pubmed: 33217323
pii: S1934-5909(20)30534-8
doi: 10.1016/j.stem.2020.10.018
pmc: PMC7875115
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

273-284.e6

Subventions

Organisme : Medical Research Council
ID : MC_PC_12009
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 203151/Z/16/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_00012/5
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_17230
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_12018/5
Pays : United Kingdom

Commentaires et corrections

Type : CommentIn

Informations de copyright

Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Interests The authors declare no competing interests.

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Auteurs

Henry De Belly (H)

MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK; Wellcome/MRC Cambridge Stem Cell Research Institute, Puddicombe Way, University of Cambridge, Cambridge CB2 0AW, UK; Department of Physiology, Development, and Neuroscience, Downing Street, University of Cambridge, Cambridge CB2 3DY, UK.

Aki Stubb (A)

Department of Physiology, Development, and Neuroscience, Downing Street, University of Cambridge, Cambridge CB2 3DY, UK.

Ayaka Yanagida (A)

Wellcome/MRC Cambridge Stem Cell Research Institute, Puddicombe Way, University of Cambridge, Cambridge CB2 0AW, UK; Living Systems Institute, University of Exeter, Exeter EX4 4QD, UK.

Céline Labouesse (C)

Wellcome/MRC Cambridge Stem Cell Research Institute, Puddicombe Way, University of Cambridge, Cambridge CB2 0AW, UK.

Philip H Jones (PH)

Department of Physics & Astronomy, University College London, Gower Street, London WC1E 6BT, UK.

Ewa K Paluch (EK)

MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK; Department of Physiology, Development, and Neuroscience, Downing Street, University of Cambridge, Cambridge CB2 3DY, UK. Electronic address: ekp25@cam.ac.uk.

Kevin J Chalut (KJ)

Wellcome/MRC Cambridge Stem Cell Research Institute, Puddicombe Way, University of Cambridge, Cambridge CB2 0AW, UK. Electronic address: kc370@cam.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Membrane Tension Gates ERK-Mediated Regulation...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Membrane Tension Gates ERK-Mediated Regulation...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches embryonnaires › Membrane Tension Gates ERK-Mediated Regulation...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Endocytose › Membrane Tension Gates ERK-Mediated Regulation...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Membrane Tension Gates ERK-Mediated Regulation...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches embryonnaires › Cellules souches embryonnaires de souris › Membrane Tension Gates ERK-Mediated Regulation...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Transduction du signal › Membrane Tension Gates ERK-Mediated Regulation...