Multiscale three-dimensional imaging of the placenta.

Confocal Imaging Light-sheet microscopy Placenta Serial block face scanning electron microscopy microCt

Journal

Placenta
ISSN: 1532-3102
Titre abrégé: Placenta
Pays: Netherlands
ID NLM: 8006349

Informations de publication

Date de publication:
12 2020
Historique:
received: 11 11 2019
revised: 30 01 2020
accepted: 31 01 2020
entrez: 21 11 2020
pubmed: 22 11 2020
medline: 15 10 2021
Statut: ppublish

Résumé

Placental function involves multiple different processes which operate at different scales from centimetres to nanometres. Everything that the placenta does from mediating blood flow to gene expression, occurs within a three-dimensional anatomical framework. This review outlines how multiscale three-dimensional imaging approaches can provide insight into placental structure and function. Three-dimensional imaging approaches include microCT, confocal, super resolution, light-sheet, and serial block-face scanning electron microscopy. Used together, these approaches allow three-dimensional imaging of the placenta across the scales at which different processes occur. Three-dimensional imaging illustrates the spatial relationships between structures and visualises structures that are not clearly apparent in two-dimensions. Understanding the three-dimensional structure of the placenta enables exploration of the relationship between structure and function, including through the development of computational models based on realistic geometries. Three-dimensional imaging approaches will enhance our understanding of placental function in health and disease.

Identifiants

pubmed: 33218580
pii: S0143-4004(20)30034-5
doi: 10.1016/j.placenta.2020.01.016
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

55-60

Informations de copyright

Copyright © 2020 Elsevier Ltd. All rights reserved.

Auteurs

Rohan M Lewis (RM)

University of Southampton, Faculty of Medicine, UK. Electronic address: rohan.lewis@soton.ac.uk.

Jennifer E Pearson-Farr (JE)

University of Southampton, Faculty of Medicine, UK.

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Classifications MeSH