A hyperelastic model for simulating cells in flow.
Atomic force microscopy
Cell deformation
Hyperelasticity
Lattice-Boltzmann
Mooney–Rivlin
Shear flow
Journal
Biomechanics and modeling in mechanobiology
ISSN: 1617-7940
Titre abrégé: Biomech Model Mechanobiol
Pays: Germany
ID NLM: 101135325
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
received:
12
02
2020
accepted:
14
10
2020
pubmed:
22
11
2020
medline:
4
11
2021
entrez:
21
11
2020
Statut:
ppublish
Résumé
In the emerging field of 3D bioprinting, cell damage due to large deformations is considered a main cause for cell death and loss of functionality inside the printed construct. Those deformations, in turn, strongly depend on the mechano-elastic response of the cell to the hydrodynamic stresses experienced during printing. In this work, we present a numerical model to simulate the deformation of biological cells in arbitrary three-dimensional flows. We consider cells as an elastic continuum according to the hyperelastic Mooney-Rivlin model. We then employ force calculations on a tetrahedralized volume mesh. To calibrate our model, we perform a series of FluidFM[Formula: see text] compression experiments with REF52 cells demonstrating that all three parameters of the Mooney-Rivlin model are required for a good description of the experimental data at very large deformations up to 80%. In addition, we validate the model by comparing to previous AFM experiments on bovine endothelial cells and artificial hydrogel particles. To investigate cell deformation in flow, we incorporate our model into Lattice Boltzmann simulations via an Immersed-Boundary algorithm. In linear shear flows, our model shows excellent agreement with analytical calculations and previous simulation data.
Identifiants
pubmed: 33219464
doi: 10.1007/s10237-020-01397-2
pii: 10.1007/s10237-020-01397-2
pmc: PMC7979664
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
509-520Références
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