Cold shock induces novel nuclear bodies in Xenopus oocytes.
Chromatin
Cold body
Nucleolus
Oocyte
Sperm
Xenopus
Journal
Experimental cell research
ISSN: 1090-2422
Titre abrégé: Exp Cell Res
Pays: United States
ID NLM: 0373226
Informations de publication
Date de publication:
01 01 2021
01 01 2021
Historique:
received:
14
09
2020
revised:
15
11
2020
accepted:
16
11
2020
pubmed:
22
11
2020
medline:
1
5
2021
entrez:
21
11
2020
Statut:
ppublish
Résumé
Here we describe novel spherical structures that are induced by cold shock on the lampbrush chromosomes (LBCs) of Xenopus laevis oocytes. We call these structures cold bodies or C-bodies. C-bodies are distributed symmetrically on homologous LBCs, with a pattern similar to that of 5S rDNA. Neither active transcription nor translation is necessary for their formation. Similar protrusions occur on the edges of some nucleoli. Endogenous LBCs as well as those derived from injected sperm form C-bodies under cold shock conditions. The function of C-bodies is unknown.
Identifiants
pubmed: 33220259
pii: S0014-4827(20)30639-X
doi: 10.1016/j.yexcr.2020.112386
pmc: PMC7771896
mid: NIHMS1652418
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
112386Subventions
Organisme : Medical Research Council
ID : MC_U137788471
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_12021/3
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : R01 GM033397
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Références
Cell. 1985 Jun;41(2):615-27
pubmed: 3986912
Exp Cell Res. 1989 Aug;183(2):508-13
pubmed: 2767163
J Cell Biol. 1995 Apr;129(2):287-98
pubmed: 7536746
Mol Biol Cell. 1998 Apr;9(4):733-47
pubmed: 9529374
J Cell Sci. 2002 May 15;115(Pt 10):2011-20
pubmed: 11973343
Nucleic Acids Res. 1988 Oct 25;16(20):9415-29
pubmed: 3141901
Methods. 2010 May;51(1):45-51
pubmed: 20060047
Mol Biol Biochem Biophys. 1986;36:1-252
pubmed: 3531807
Mol Biol Cell. 1999 Dec;10(12):4385-402
pubmed: 10588665
Methods Cell Biol. 1991;36:149-66
pubmed: 1811131
Mol Biol Cell. 2002 Oct;13(10):3466-76
pubmed: 12388750
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
Chromosome Res. 2012 Dec;20(8):971-8
pubmed: 23296495
Science. 1968 Apr 19;160(3825):272-80
pubmed: 4867987
Exp Cell Res. 2004 May 15;296(1):28-34
pubmed: 15120990
Biol Cell. 1990;69(3):139-51
pubmed: 2097000
J Exp Zool. 1973 Jun;184(3):321-33
pubmed: 4708138
Genetics. 1969;61(1):Suppl:121-32
pubmed: 5345375
Proc Natl Acad Sci U S A. 1968 Jun;60(2):553-60
pubmed: 5248812