The Effect of Allograft Transplantation of Sertoli Cell on Expression of NF-кB, Bax Proteins, and Ischemic Tolerance in Rats with Focal Cerebral Ischemia.

Apoptosis Blood-brain barrier Brain ischemia Cell transplantation Edema Infarction Inflammation

Journal

Iranian journal of pharmaceutical research : IJPR
ISSN: 1735-0328
Titre abrégé: Iran J Pharm Res
Pays: Netherlands
ID NLM: 101208407

Informations de publication

Date de publication:
2020
Historique:
entrez: 23 11 2020
pubmed: 24 11 2020
medline: 24 11 2020
Statut: ppublish

Résumé

One of the newest methods to reduce cerebral ischemia damages is cell therapy. The aim of this study is to evaluate the effect of Sertoli cell transplantation on ischemia-induced injuries in animal models of stroke. Rats were divided into four groups: transplant+ischemia, ischemia, sham, and control. Sertoli cells were separated from the other testis of rats and cultured. Unilateral Sertoli cell transplantation was performed in the right striatum by using stereotaxic surgery. For induction of brain ischemia, middle cerebral artery occlusion surgery was used 14 days after transplantation. By using western blotting method, expression of nuclear factor kappa (NF-кB) and Bax were evaluated. In this study, a remarkable decrease in neurological deficits, infection, blood-brain barrier permeability, and brain edema was observed in the cell transplant recipient group in comparison with the ischemia group. Probably, a reduction in inflammation (NF-кB factor) and apoptosis (Bax) following injection of Sertoli cells result in amelioration of ischemic damages induced by MCAO surgery.

Identifiants

pubmed: 33224215
doi: 10.22037/ijpr.2020.15574.13189
pmc: PMC7667533
doi:

Types de publication

Journal Article

Langues

eng

Pagination

98-114

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Auteurs

Roya Hosseini (R)

Department of Physiology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.

Mohammad Reza Bigdeli (MR)

Department of Physiology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Institute for Cognitive and Brain Sciences, Shahid Beheshti University, Tehran, Iran.

Sepideh Khaksar (S)

Department of Plant Sciences, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.

Abbas Aliaghaei (A)

Department of Anatomy and Cell Biology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Classifications MeSH