Comparison of sperm selection techniques in donkeys: motile subpopulations from a practical point of view.
SLC
cryopreservation
subpopulations
Journal
Animal reproduction
ISSN: 1984-3143
Titre abrégé: Anim Reprod
Pays: Brazil
ID NLM: 101272804
Informations de publication
Date de publication:
23 Oct 2019
23 Oct 2019
Historique:
entrez:
23
11
2020
pubmed:
24
11
2020
medline:
24
11
2020
Statut:
epublish
Résumé
The aim of this study was to compare the post-thaw distribution of motile sperm subpopulations, following simple or colloid centrifugation. A new analysis was used to evaluate the available number of sperm from each subpopulation after each centrifugation protocol. Frozen/thawed semen samples were divided into the following after-thawing treatments: uncentrifuged control (UDC), sperm washing (SW) and two colloid centrifugation procedures (Equipure, SLC-E, and Androcoll, SLC-A). Percentage of total and progressive motility (TM and PM), as well as sperm motility kinematics, distribution of motile sperm subpopulations, and recovery rates, were statistically compared among treatments. The SLC treatments showed higher (P < 0.001) TM and PM than UDC and SW. Following each SLC procedure, different percentages of the subpopulation with the most vigorous and progressive sperm (sP4) were obtained. SLC-A recovered a larger number of sperm belonging to sP4 than SLC-E, but not significantly higher than SW. From a practical point of view, sperm washing, the standard centrifugation procedure for equine semen processing, recovered the same amount of fast and progressive sperm as colloid centrifugation, apparently the best treatment according to traditional analysis. In conclusion, samples processed by SLC have higher motility percentages than SW and UDC but, after combining the available number of sperm, SLC and SW techniques are equally efficient in recovering sperm from the most vigorous, fast and progressive motile subpopulation (sP4).
Identifiants
pubmed: 33224288
doi: 10.21451/1984-3143-AR2018-0133
pii: ar-v16n2-p282
pmc: PMC7673585
doi:
Types de publication
Journal Article
Langues
eng
Pagination
282-289Informations de copyright
Copyright © The Author(s). Published by CBRA.
Déclaration de conflit d'intérêts
Conflict of interest
The authors declare that there is no conflict of interest regarding the publication of this article.
Références
Theriogenology. 2009 Nov;72(8):1017-22
pubmed: 19747718
Reprod Domest Anim. 2003 Aug;38(4):305-11
pubmed: 12887569
Theriogenology. 2018 Jul 15;115:99-107
pubmed: 29747159
J Androl. 1996 May-Jun;17(3):183-6
pubmed: 8792208
Reprod Domest Anim. 2009 Jun;44(3):419-23
pubmed: 19055563
Hum Reprod. 2000 Jun;15(6):1322-8
pubmed: 10831563
Anim Reprod Sci. 2013 Dec;143(1-4):118-25
pubmed: 24210910
Anim Reprod Sci. 2009 Nov;116(1-2):119-28
pubmed: 19217226
Reprod Fertil Dev. 2015 Jan;27(2):332-40
pubmed: 25482321
J Anim Sci Biotechnol. 2017 Mar 7;8:23
pubmed: 28286648
Anim Reprod Sci. 2013 Dec;143(1-4):64-71
pubmed: 24210876
Anim Reprod Sci. 2013 Jul;140(1-2):62-9
pubmed: 23778304
Theriogenology. 2012 Nov;78(8):1846-54
pubmed: 22979965
Theriogenology. 2011 Oct 15;76(7):1177-86
pubmed: 21835453
Reprod Domest Anim. 2009 Jun;44(3):523-6
pubmed: 18992085
Animal. 2014 Feb;8(2):308-15
pubmed: 24284302
Anim Reprod Sci. 2013 Aug;140(3-4):173-9
pubmed: 23890802
Theriogenology. 2013 Nov;80(8):955-62
pubmed: 23987984
Reprod Domest Anim. 2008 Aug;43(4):393-9
pubmed: 18282216
Theriogenology. 2014 May;81(8):1005-11
pubmed: 24582376
Theriogenology. 2016 Apr 15;85(7):1267-73
pubmed: 26806444
Theriogenology. 2015 Jul 15;84(2):294-300
pubmed: 25917884
Biol Reprod. 2005 Feb;72(2):316-27
pubmed: 15385419
Anim Reprod Sci. 2015 Jul;158:68-74
pubmed: 26002696
Anim Reprod Sci. 2017 Oct;185:83-88
pubmed: 28827080
Theriogenology. 2010 Sep 15;74(5):795-804
pubmed: 20673991
Theriogenology. 2008 Oct 1;70(6):936-45
pubmed: 18602684
Anim Reprod Sci. 2018 Sep;196:48-58
pubmed: 30041960
Theriogenology. 2009 Oct 1;72(6):860-8
pubmed: 19640577
Saudi J Biol Sci. 2017 Nov;24(7):1489-1496
pubmed: 30294217
Acta Vet Scand. 2012 Jul 12;54:40
pubmed: 22788670
Anim Reprod Sci. 2009 Aug;114(1-3):193-202
pubmed: 18842364