dSTORM Imaging and Analysis of Desmosome Architecture.
Cell junctions
Microscopy
STORM
Super-resolution
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
pubmed:
24
11
2020
medline:
13
1
2022
entrez:
23
11
2020
Statut:
ppublish
Résumé
Desmosomes are cell-cell junctions responsible for mechanically integrating adjacent cells. Due to the small size of the junctions, their protein architecture cannot be elucidated using conventional fluorescence microscopy. Super-resolution microscopy techniques, including dSTORM, deliver higher-resolution images which can reveal the localization or arrangement of proteins within individual desmosomes. Herein we describe an imaging and analysis method to determine the nanoscale architecture of desmosomes using super-resolution dSTORM.
Identifiants
pubmed: 33225407
doi: 10.1007/7651_2020_325
doi:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
305-315Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR072697
Pays : United States
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