Role Of Alteration Of Ck5\6 Profile In Dysplastic Progression Of Oral Mucosa In Tobacco Users.
Oral dysplastic lesions; CK5\6
Oral squamous cell carcinoma
Journal
Journal of Ayub Medical College, Abbottabad : JAMC
ISSN: 1819-2718
Titre abrégé: J Ayub Med Coll Abbottabad
Pays: Pakistan
ID NLM: 8910750
Informations de publication
Date de publication:
Historique:
entrez:
23
11
2020
pubmed:
24
11
2020
medline:
6
1
2021
Statut:
ppublish
Résumé
To determine staining expression of CK5\6 in healthy oral mucosa and various grades of oral dysplasia and to find out possible association of CK5\6 expression in dysplastic transformation of clinically normal oral mucosa to various grades of oral dysplasia. This cross-sectional descriptive study was done at Al-Tibri Medical College and Hospital and Dow University of Health Sciences, Karachi from March 2018 to November 2018. It included 120 diagnosed paraffin embedded tissue samples of normal oral mucosa and various grades of oral epithelial dysplastic lesions. Patient's data was reviewed for age, gender and tobacco habits. For immunohistochemistry CK5\6 staining was performed on all the samples. Immunohistochemical evaluation was done by observing the staining expression of CK5\6 on various oral dysplastic samples on the basis of staining intensity. The compiled data was statistically analyzed by using Chi-square. p-values of <0.05 were considered to be significant. All of the 60/60 (100%) oral dysplastic cases were moderately to strongly positive for CK5\6. Gradual increase in staining intensity for CK5\6 was observed with increasing grades of dysplasia. We found highly significant association of CK5\6 immunopositivity in transformation of normal mucosa to various grades oral dysplastic lesions. CK5\6 can be used as reliable adjuvant marker for the early dysplastic transformation of oral mucosain tobacco users, before it progresses to oral squamous cell carcinoma (OSCC).
Sections du résumé
BACKGROUND
BACKGROUND
To determine staining expression of CK5\6 in healthy oral mucosa and various grades of oral dysplasia and to find out possible association of CK5\6 expression in dysplastic transformation of clinically normal oral mucosa to various grades of oral dysplasia.
METHODS
METHODS
This cross-sectional descriptive study was done at Al-Tibri Medical College and Hospital and Dow University of Health Sciences, Karachi from March 2018 to November 2018. It included 120 diagnosed paraffin embedded tissue samples of normal oral mucosa and various grades of oral epithelial dysplastic lesions. Patient's data was reviewed for age, gender and tobacco habits. For immunohistochemistry CK5\6 staining was performed on all the samples. Immunohistochemical evaluation was done by observing the staining expression of CK5\6 on various oral dysplastic samples on the basis of staining intensity. The compiled data was statistically analyzed by using Chi-square. p-values of <0.05 were considered to be significant.
RESULTS
RESULTS
All of the 60/60 (100%) oral dysplastic cases were moderately to strongly positive for CK5\6. Gradual increase in staining intensity for CK5\6 was observed with increasing grades of dysplasia. We found highly significant association of CK5\6 immunopositivity in transformation of normal mucosa to various grades oral dysplastic lesions.
CONCLUSIONS
CONCLUSIONS
CK5\6 can be used as reliable adjuvant marker for the early dysplastic transformation of oral mucosain tobacco users, before it progresses to oral squamous cell carcinoma (OSCC).
Substances chimiques
Keratin-5
0
Keratin-6
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM