High resolution ultrasound imaging for repeated measure of wound tissue morphometry, biomechanics and hemodynamics under fetal, adult and diabetic conditions.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
12
12
2019
accepted:
21
10
2020
entrez:
23
11
2020
pubmed:
24
11
2020
medline:
21
1
2021
Statut:
epublish
Résumé
Non-invasive, repeated interrogation of the same wound is necessary to understand the tissue repair continuum. In this work, we sought to test the significance of non-invasive high-frequency high-resolution ultrasound technology for such interrogation. High-frequency high-resolution ultrasound imaging was employed to investigate wound healing under fetal and adult conditions. Quantitative tissue cellularity and elastic strain was obtained for visualization of unresolved inflammation using Vevo strain software. Hemodynamic properties of the blood flow in the artery supplying the wound-site were studied using color Doppler flow imaging. Non-invasive monitoring of fetal and adult wound healing provided unprecedented biomechanical and functional insight. Fetal wounds showed highly accelerated closure with transient perturbation of wound tissue cellularity. Fetal hemodynamics was unique in that sharp fall in arterial pulse pressure (APP) which was rapidly restored within 48h post-wounding. In adults, APP transiently increased post-wounding before returning to the pre-wounding levels by d10 post-wounding. The pattern of change in the elasticity of wound-edge tissue of diabetics was strikingly different. Severe strain acquired during the early inflammatory phase persisted with a slower recovery of elasticity compared to that of the non-diabetic group. Wound bed of adult diabetic mice (db/db) showed persistent hypercellularity compared to littermate controls (db/+) indicative of prolonged inflammation. Normal skin strain of db/+ and db/db were asynchronous. In db/db, severe strain acquired during the early inflammatory phase persisted with a slower recovery of elasticity compared to that of non-diabetics. This study showcases a versatile clinically relevant imaging platform suitable for real-time analyses of functional wound healing.
Identifiants
pubmed: 33227015
doi: 10.1371/journal.pone.0241831
pii: PONE-D-19-34429
pmc: PMC7682876
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0241831Subventions
Organisme : NINDS NIH HHS
ID : R01 NS042617
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM108014
Pays : United States
Organisme : NINR NIH HHS
ID : R01 NR015676
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK114718
Pays : United States
Organisme : NINR NIH HHS
ID : R01 NR013898
Pays : United States
Organisme : NIDDK NIH HHS
ID : U01 DK119099
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM077185
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK125835
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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