Sex-dimorphic Rostro-caudal Patterns of 5'-AMP-activated Protein Kinase Activation and Glucoregulatory Transmitter Marker Protein Expression in the Ventrolateral Ventromedial Hypothalamic Nucleus (VMNvl) in Hypoglycemic Male and Female Rats: Impact of Estradiol.
AMP-Activated Protein Kinase Kinases
Animals
Estradiol
/ metabolism
Female
Glucose
/ metabolism
Glutamate Decarboxylase
/ genetics
Hypoglycemia
/ metabolism
Hypothalamus
/ drug effects
Insulin
/ metabolism
Male
Nitric Oxide Synthase Type I
/ genetics
Protein Kinases
/ genetics
RNA Splicing Factors
/ genetics
Rats
Rats, Sprague-Dawley
Signal Transduction
Glutamate decarboxylase
Insulin-induced hypoglycemia
Letrozole
PhosphoAMPK
Ventrolateral ventromedial hypothalamic nucleus-ventrolateral part
Journal
Journal of molecular neuroscience : MN
ISSN: 1559-1166
Titre abrégé: J Mol Neurosci
Pays: United States
ID NLM: 9002991
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
received:
18
08
2020
accepted:
07
10
2020
pubmed:
25
11
2020
medline:
20
11
2021
entrez:
24
11
2020
Statut:
ppublish
Résumé
The ventromedial hypothalamic nucleus-ventrolateral part (VMNvl) is an estradiol-sensitive structure that controls sex-specific behavior. Electrical reactivity of VMNvl neurons to hypoglycemia infers that cellular energy stability is monitored there. Current research investigated the hypothesis that estradiol elicits sex-dimorphic patterns of VMNvl metabolic sensor activation and gluco-regulatory neurotransmission during hypoglycemia. Rostral-, middle-, and caudal-VMNvl tissue was separately micropunch-dissected from letrozole (Lz)- or vehicle-injected male and estradiol- or vehicle-implanted ovariectomized (OVX) female rats for Western blot analysis of total and phosphorylated 5'-AMP-activated protein kinase (AMPK) protein expression and gluco-stimulatory [neuronal nitric oxide synthase (nNOS); steroidogenic factor-1 (SF1) or -inhibitory (glutamate decarboxylase
Identifiants
pubmed: 33231812
doi: 10.1007/s12031-020-01730-5
pii: 10.1007/s12031-020-01730-5
pmc: PMC8076060
mid: NIHMS1650402
doi:
Substances chimiques
Insulin
0
RNA Splicing Factors
0
Sf1 protein, rat
0
Estradiol
4TI98Z838E
Nitric Oxide Synthase Type I
EC 1.14.13.39
Protein Kinases
EC 2.7.-
AMP-Activated Protein Kinase Kinases
EC 2.7.11.3
Glutamate Decarboxylase
EC 4.1.1.15
glutamate decarboxylase 1
EC 4.1.1.15
glutamate decarboxylase 2
EC 4.1.1.15
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1082-1094Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK109382
Pays : United States
Organisme : NIDDK NIH HHS
ID : DK 109382
Pays : United States
Organisme : NIDDK NIH HHS
ID : DK 109382
Pays : United States
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