Proteomic analysis of young and old mouse hematopoietic stem cells and their progenitors reveals post-transcriptional regulation in stem cells.
hematopoiesis
mass spectromtetry
mouse
proteomics
regenerative medicine
stem cell
stem cells
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
25 11 2020
25 11 2020
Historique:
received:
18
08
2020
accepted:
16
11
2020
entrez:
25
11
2020
pubmed:
26
11
2020
medline:
2
4
2021
Statut:
epublish
Résumé
The balance of hematopoietic stem cell (HSC) self-renewal and differentiation is critical for a healthy blood supply; imbalances underlie hematological diseases. The importance of HSCs and their progenitors have led to their extensive characterization at genomic and transcriptomic levels. However, the proteomics of hematopoiesis remains incompletely understood. Here we report a proteomics resource from mass spectrometry of mouse young adult and old adult mouse HSCs, multipotent progenitors and oligopotent progenitors; 12 cell types in total. We validated differential protein levels, including confirmation that Dnmt3a protein levels are undetected in young adult mouse HSCs until forced into cycle. Additionally, through integrating proteomics and RNA-sequencing datasets, we identified a subset of genes with apparent post-transcriptional repression in young adult mouse HSCs. In summary, we report proteomic coverage of young and old mouse HSCs and progenitors, with broader implications for understanding mechanisms for stem cell maintenance, niche interactions and fate determination.
Identifiants
pubmed: 33236985
doi: 10.7554/eLife.62210
pii: 62210
pmc: PMC7688314
doi:
pii:
Substances chimiques
Proteome
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NCI NIH HHS
ID : R35 CA220434
Pays : United States
Organisme : NCI NIH HHS
ID : CA09302
Pays : United States
Organisme : NHLBI NIH HHS
ID : T32 HL120824
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK115600
Pays : United States
Informations de copyright
© 2020, Zaro et al.
Déclaration de conflit d'intérêts
BZ, JN, VM, JD, BG, MZ, GG, RS, RF, AB, AZ, AW, PJ, IW No competing interests declared
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