Influence of image analysis strategy, cooling rate, and sample volume on apparent protein cloud-point temperature determination.

Colloidal stability Freezing High-throughput screening Liquid–liquid phase separation Nucleation temperature Protein stability

Journal

Bioprocess and biosystems engineering
ISSN: 1615-7605
Titre abrégé: Bioprocess Biosyst Eng
Pays: Germany
ID NLM: 101088505

Informations de publication

Date de publication:
Mar 2021
Historique:
received: 24 06 2020
accepted: 16 10 2020
pubmed: 26 11 2020
medline: 29 10 2021
entrez: 25 11 2020
Statut: ppublish

Résumé

The protein cloud-point temperature (T

Identifiants

pubmed: 33237399
doi: 10.1007/s00449-020-02465-8
pii: 10.1007/s00449-020-02465-8
pmc: PMC7889528
doi:

Substances chimiques

Muramidase EC 3.2.1.17

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

525-536

Références

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pubmed: 25084371
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Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):377-82
pubmed: 8552642

Auteurs

Marieke E Klijn (ME)

Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 2, 76131, Karlsruhe, Germany.

Jürgen Hubbuch (J)

Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 2, 76131, Karlsruhe, Germany. juergen.hubbuch@kit.edu.

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Classifications MeSH