An in vitro ovarian explant culture system to examine sex change in a hermaphroditic fish.

Cortisol Organ culture Previtellogenic oocyte Sex change Spotty wrasse

Journal

PeerJ
ISSN: 2167-8359
Titre abrégé: PeerJ
Pays: United States
ID NLM: 101603425

Informations de publication

Date de publication:
2020
Historique:
received: 20 05 2020
accepted: 19 10 2020
entrez: 26 11 2020
pubmed: 27 11 2020
medline: 27 11 2020
Statut: epublish

Résumé

Many teleost fishes undergo natural sex change, and elucidating the physiological and molecular controls of this process offers unique opportunities not only to develop methods of controlling sex in aquaculture settings, but to better understand vertebrate sexual development more broadly. Induction of sex change in some sequentially hermaphroditic or gonochoristic fish can be achieved in vivo through social manipulation, inhibition of aromatase activity, or steroid treatment. However, the induction of sex change in vitro has been largely unexplored. In this study, we established an in vitro culture system for ovarian explants in serum-free medium for a model sequential hermaphrodite, the New Zealand spotty wrasse (

Identifiants

pubmed: 33240644
doi: 10.7717/peerj.10323
pii: 10323
pmc: PMC7666549
doi:

Types de publication

Journal Article

Langues

eng

Pagination

e10323

Informations de copyright

©2020 Goikoetxea et al.

Déclaration de conflit d'intérêts

The authors declare there are no competing interests.

Références

Mol Reprod Dev. 2010 Aug;77(8):679-86
pubmed: 20653000
Gen Comp Endocrinol. 2010 May 15;167(1):44-50
pubmed: 20178798
Mol Reprod Dev. 2012 Oct;79(10):719-26
pubmed: 22888054
Endocrinology. 2010 Aug;151(8):3900-8
pubmed: 20534725
Gen Comp Endocrinol. 1986 Nov;64(2):312-9
pubmed: 3557096
Gen Comp Endocrinol. 1978 Jul;35(3):322-8
pubmed: 689364
Sex Dev. 2015;9(2):118-24
pubmed: 25676411
Gen Comp Endocrinol. 2019 Sep 1;280:123-133
pubmed: 31009604
Curr Top Dev Biol. 2019;134:71-117
pubmed: 30999982
Methods. 2016 Mar 1;96:6-11
pubmed: 26687239
Proc Natl Acad Sci U S A. 1991 Jul 1;88(13):5774-8
pubmed: 2062857
Curr Opin Genet Dev. 2008 Dec;18(6):499-505
pubmed: 19152784
PeerJ. 2019 Jun 11;7:e7032
pubmed: 31218121
Gen Comp Endocrinol. 2010 Feb 1;165(3):390-411
pubmed: 19348807
J Exp Zool A Ecol Genet Physiol. 2012 Nov;317(9):552-60
pubmed: 22927221
Zoolog Sci. 2006 Jan;23(1):65-9
pubmed: 16547407
Gen Comp Endocrinol. 1990 Mar;77(3):403-7
pubmed: 2338220
Sex Dev. 2016;10(5-6):223-241
pubmed: 27820936
Front Endocrinol (Lausanne). 2020 Jan 31;11:12
pubmed: 32082256
Comp Biochem Physiol A Mol Integr Physiol. 2019 Jan;227:154-160
pubmed: 30326270
Mol Reprod Dev. 2017 Feb;84(2):171-194
pubmed: 27543780
Gen Comp Endocrinol. 2013 Oct 1;192:36-44
pubmed: 23770022
Endocrinology. 2012 Dec;153(12):6003-11
pubmed: 23041673
Semin Cell Dev Biol. 2009 May;20(3):264-70
pubmed: 19124081
Integr Comp Biol. 2015 Aug;55(2):307-22
pubmed: 25980565
Reproduction. 2017 Dec;154(6):R149-R160
pubmed: 28890443
Endocrinology. 2006 Nov;147(11):5139-46
pubmed: 16887910

Auteurs

Alexander Goikoetxea (A)

Department of Anatomy, University of Otago, Dunedin, New Zealand.

Erin L Damsteegt (EL)

Department of Zoology, University of Otago, Dunedin, New Zealand.

Erica V Todd (EV)

School of Life and Environmental Science, Deakin University, Geelong, Australia.

Andrew McNaughton (A)

Department of Anatomy, University of Otago, Dunedin, New Zealand.

Neil J Gemmell (NJ)

Department of Anatomy, University of Otago, Dunedin, New Zealand.

P Mark Lokman (PM)

Department of Zoology, University of Otago, Dunedin, New Zealand.

Classifications MeSH