Determination of Chloramphenicol and Nitrofuran Metabolites in Cobia, Croaker, and Shrimp Using Microwave-Assisted Derivatization, Automated SPE, and LC-MS/MS-Results from a U.S. Food and Drug Administration Level Three Inter-Laboratory Study.
Journal
Journal of AOAC International
ISSN: 1944-7922
Titre abrégé: J AOAC Int
Pays: England
ID NLM: 9215446
Informations de publication
Date de publication:
01 Jul 2020
01 Jul 2020
Historique:
received:
01
10
2019
revised:
23
01
2020
accepted:
06
02
2020
entrez:
26
11
2020
pubmed:
27
11
2020
medline:
29
6
2021
Statut:
ppublish
Résumé
An interlaboratory study was conducted to test a published, peer-reviewed manuscript in the Journal of AOAC INTERNATIONAL Vol 98, No. 3, 2015, "Quantitation of Chloramphenicol and Nitrofuran Metabolites in Aquaculture Products Using Microwave-Assisted Derivatization, Automated Solid-Phase Extraction, and LC-MS/MS." The purpose of this study was to demonstrate the performance of the method in shrimp, cobia, and croaker matrices. Three U.S. Food and Drug Administration laboratories participated in the collaborative study. The laboratories tested matrix blanks and laboratory-fortified matrix blanks at various levels in three separate matrices. The method evaluation included determination of the LOQ, accuracy, and precision. The reproducibility and repeatability of the RSD, % levels for matrix spikes fortified below the action level were < 10% for all residues in shrimp, < 13% for all residues in cobia except for 3-amino-2-oxazolidinone which was ≤ 22%, and < 16% for croaker. The RSD, % levels for all other spikes in the study were < 14%. Average percent recoveries for all matrices ranged from 81.6% - 102%. The study demonstrated that the method is acceptable for use for the combined determination of chloramphenicol and nitrofuran metabolites in the study matrices. The study showed acceptable quantitation for the high-throughput chloramphenicol and nitrofuran metabolites method.
Sections du résumé
BACKGROUND
BACKGROUND
An interlaboratory study was conducted to test a published, peer-reviewed manuscript in the Journal of AOAC INTERNATIONAL Vol 98, No. 3, 2015, "Quantitation of Chloramphenicol and Nitrofuran Metabolites in Aquaculture Products Using Microwave-Assisted Derivatization, Automated Solid-Phase Extraction, and LC-MS/MS."
OBJECTIVE
OBJECTIVE
The purpose of this study was to demonstrate the performance of the method in shrimp, cobia, and croaker matrices.
METHOD
METHODS
Three U.S. Food and Drug Administration laboratories participated in the collaborative study. The laboratories tested matrix blanks and laboratory-fortified matrix blanks at various levels in three separate matrices. The method evaluation included determination of the LOQ, accuracy, and precision.
RESULTS
RESULTS
The reproducibility and repeatability of the RSD, % levels for matrix spikes fortified below the action level were < 10% for all residues in shrimp, < 13% for all residues in cobia except for 3-amino-2-oxazolidinone which was ≤ 22%, and < 16% for croaker. The RSD, % levels for all other spikes in the study were < 14%. Average percent recoveries for all matrices ranged from 81.6% - 102%.
CONCLUSIONS
CONCLUSIONS
The study demonstrated that the method is acceptable for use for the combined determination of chloramphenicol and nitrofuran metabolites in the study matrices.
HIGHLIGHTS
CONCLUSIONS
The study showed acceptable quantitation for the high-throughput chloramphenicol and nitrofuran metabolites method.
Identifiants
pubmed: 33241344
pii: 5851561
doi: 10.1093/jaoacint/qsaa019
doi:
Substances chimiques
Nitrofurans
0
Chloramphenicol
66974FR9Q1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1043-1051Investigateurs
N Bandara
(N)
B Broadaway
(B)
C Casey
(C)
A Fong
(A)
A Karmakar
(A)
M Mayweather
(M)
A Morara
(A)
N Williams
(N)
B Wright
(B)
L Ye
(L)
Informations de copyright
AOAC INTERNATIONAL 2020. This work is written by a US Government employee and is in the public domain in the US.