Lassa hemorrhagic shock syndrome-on-a-chip.
Lassa hemorrhagic fever
Organ Chips
drug
microvessel
vascular permeability
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
07
10
2020
revised:
17
11
2020
accepted:
23
11
2020
pubmed:
27
11
2020
medline:
18
1
2022
entrez:
26
11
2020
Statut:
ppublish
Résumé
Lack of experimental human models hinders research on Lassa hemorrhagic fever and the development of treatment strategies. Here, we report the first chip-based model for Lassa hemorrhagic syndrome. The chip features a microvessel interfacing collagen network as a simple mimic for extracellular matrix, allowing for quantitative and real-time vascular integrity assessment. Luminal infusion of Lassa virus-like particles led to a dramatic increase in vascular permeability in a viral load-dependent manner. Using this platform, we showed that Fibrin-derived peptide FX06 can be used to suppress the vascular integrity loss. This simple chip-based model proved promising in the assessment of disease severity and provides an easy-to-use platform for future investigation of Lassa pathogenesis and drug development in a human-like setting.
Identifiants
pubmed: 33241859
doi: 10.1002/bit.27636
pmc: PMC7983903
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1405-1410Informations de copyright
© 2020 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals LLC.
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