Tools for the targeted genetic modification of poxvirus genomes.
Journal
Current opinion in virology
ISSN: 1879-6265
Titre abrégé: Curr Opin Virol
Pays: Netherlands
ID NLM: 101560941
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
02
10
2020
revised:
27
10
2020
accepted:
27
10
2020
pubmed:
27
11
2020
medline:
26
8
2021
entrez:
26
11
2020
Statut:
ppublish
Résumé
The potential of viruses as biotechnology platforms is becoming more appealing due to technological advances in synthetic biology techniques and to the increasing accessibility of means to manipulate virus genomes. Among viral systems, poxviruses, and their prototype member Vaccinia Virus, are one of the outstanding choices for different biotechnological and medical applications based on heterologous gene expression, recombinant vaccines or oncolytic viruses. The refinement of genetic engineering methods on Vaccinia Virus over the last decades have contributed to facilitate the manipulation of the genomes of poxviruses, and may aid in the improvement of virus variants designed for different goals through reverse genetic approaches. Targeted genetic changes are usually performed by homologous recombination with the viral genome. In addition to the classic approach, recent methodological advances that may assist new strategies for the mutation or edition of poxvirus genomes are reviewed.
Identifiants
pubmed: 33242829
pii: S1879-6257(20)30109-7
doi: 10.1016/j.coviro.2020.10.006
pii:
doi:
Substances chimiques
Vaccines, Synthetic
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
183-190Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.