Elevated L-threonine is a biomarker for Lassa fever and Ebola.
Ebola
L-Threonine
Lassa fever
Liquid Chromatography Mass Spectrometry
Metabolomics
Viral hemorrhagic fevers
Journal
Virology journal
ISSN: 1743-422X
Titre abrégé: Virol J
Pays: England
ID NLM: 101231645
Informations de publication
Date de publication:
26 11 2020
26 11 2020
Historique:
received:
02
10
2020
accepted:
23
11
2020
entrez:
27
11
2020
pubmed:
28
11
2020
medline:
20
8
2021
Statut:
epublish
Résumé
Lassa fever and Ebola are characterized by non-specific initial presentations that can progress to severe multisystem illnesses with high fatality rates. Samples from additional subjects are examined to extend and corroborate biomarkers with prognostic value for these diseases. Liquid Chromatography Mass Spectrometry metabolomics was used to identify and confirm metabolites disrupted in the blood of Lassa fever and Ebola patients. Authenticated standards are used to confirm the identify of key metabolites. We confirm prior results by other investigators that the amino acid L-threonine is elevated during Ebola virus infection. L-Threonine is also elevated during Lassa virus infection. We also confirmed that platelet-activating factor (PAF) and molecules with PAF moiety are reduced in the blood of patients with fatal Lassa fever. Similar changes in PAF and PAF-like molecules were not observed in the blood of Ebola patients. Metabolomics may provide tools to identify pathways that are differentially affected during viral hemorrhagic fevers and guide development of diagnostics to monitor and predict outcome.
Sections du résumé
BACKGROUND
Lassa fever and Ebola are characterized by non-specific initial presentations that can progress to severe multisystem illnesses with high fatality rates. Samples from additional subjects are examined to extend and corroborate biomarkers with prognostic value for these diseases.
METHODS
Liquid Chromatography Mass Spectrometry metabolomics was used to identify and confirm metabolites disrupted in the blood of Lassa fever and Ebola patients. Authenticated standards are used to confirm the identify of key metabolites.
RESULTS
We confirm prior results by other investigators that the amino acid L-threonine is elevated during Ebola virus infection. L-Threonine is also elevated during Lassa virus infection. We also confirmed that platelet-activating factor (PAF) and molecules with PAF moiety are reduced in the blood of patients with fatal Lassa fever. Similar changes in PAF and PAF-like molecules were not observed in the blood of Ebola patients.
CONCLUSIONS
Metabolomics may provide tools to identify pathways that are differentially affected during viral hemorrhagic fevers and guide development of diagnostics to monitor and predict outcome.
Identifiants
pubmed: 33243278
doi: 10.1186/s12985-020-01459-y
pii: 10.1186/s12985-020-01459-y
pmc: PMC7690152
doi:
Substances chimiques
Biomarkers
0
Threonine
2ZD004190S
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
188Subventions
Organisme : NIAID NIH HHS
ID : U19 AI135995
Pays : United States
Organisme : National Institute of Allergy and Infectious Diseases
ID : AI115754
Organisme : National Institute of Allergy and Infectious Diseases
ID : AI104621
Organisme : National Institute of Allergy and Infectious Diseases
ID : AI114855
Organisme : National Institute of Allergy and Infectious Diseases
ID : 1U19AI135995
Références
PLoS One. 2012;7(7):e40221
pubmed: 22844400
Malar J. 2017 May 30;16(1):229
pubmed: 28558710
Br J Haematol. 1989 Aug;72(4):543-8
pubmed: 2775659
PLoS Negl Trop Dis. 2017 Sep 18;11(9):e0005943
pubmed: 28922385
Antiviral Res. 2008 Apr;78(1):103-15
pubmed: 18241935
Cell Host Microbe. 2017 Dec 13;22(6):817-829.e8
pubmed: 29154144
Sci Rep. 2016 Nov 15;6:36833
pubmed: 27845374
Am J Trop Med Hyg. 1985 Sep;34(5):999-1007
pubmed: 4037187
Clin Chim Acta. 2016 Sep 1;460:23-32
pubmed: 27288986