The pulmonary toxicity of carboxylated or aminated multi-walled carbon nanotubes in mice is determined by the prior purification method.
Carbon nanotubes
Fibrosis
Functionalization
Lung injury
Purification
Journal
Particle and fibre toxicology
ISSN: 1743-8977
Titre abrégé: Part Fibre Toxicol
Pays: England
ID NLM: 101236354
Informations de publication
Date de publication:
26 11 2020
26 11 2020
Historique:
received:
03
07
2020
accepted:
11
11
2020
entrez:
27
11
2020
pubmed:
28
11
2020
medline:
25
6
2021
Statut:
epublish
Résumé
Inhalation of multi-walled carbon nanotubes (MWCNTs) poses a potential risk to human health. In order to safeguard workers and consumers, the toxic properties of MWCNTs need to be identified. Functionalization has been shown to either decrease or increase MWCNT-related pulmonary injury, depending on the type of modification. We, therefore, investigated both acute and chronic pulmonary toxicity of a library of MWCNTs derived from a common pristine parent compound (NC7000). MWCNTs were thermally or chemically purified and subsequently surface functionalized by carboxylation or amination. To evaluate pulmonary toxicity, male C57BL6 mice were dosed via oropharyngeal aspiration with either 1.6 or 4 mg/kg of each MWCNT type. Mitsui-7 MWCNT was used as a positive control. Necropsy was performed at days 3 and 60 post-exposure to collect bronchoalveolar lavage fluid (BALF) and lungs. At day 3 all MWCNTs increased the number of neutrophils in BALF. Chemical purification had a greater effect on pro-inflammatory cytokines (IL-1β, IL-6, CXCL1) in BALF, while thermal purification had a greater effect on pro-fibrotic cytokines (CCL2, OPN, TGF-β1). At day 60, thermally purified, carboxylated MWCNTs had the strongest effect on lymphocyte numbers in BALF. Thermally purified MWCNTs caused the greatest increase in LDH and total protein in BALF. Furthermore, the thermally purified and carboxyl- or amine-functionalized MWCNTs caused the greatest number of granulomatous lesions in the lungs. The physicochemical characteristics mainly associated with increased toxicity of the thermally purified derivatives were decreased surface defects and decreased amorphous content as indicated by Raman spectroscopy. These data demonstrate that the purification method is an important determinant of lung toxicity induced by carboxyl- and amine-functionalized MWCNTs.
Sections du résumé
BACKGROUND
Inhalation of multi-walled carbon nanotubes (MWCNTs) poses a potential risk to human health. In order to safeguard workers and consumers, the toxic properties of MWCNTs need to be identified. Functionalization has been shown to either decrease or increase MWCNT-related pulmonary injury, depending on the type of modification. We, therefore, investigated both acute and chronic pulmonary toxicity of a library of MWCNTs derived from a common pristine parent compound (NC7000).
METHODS
MWCNTs were thermally or chemically purified and subsequently surface functionalized by carboxylation or amination. To evaluate pulmonary toxicity, male C57BL6 mice were dosed via oropharyngeal aspiration with either 1.6 or 4 mg/kg of each MWCNT type. Mitsui-7 MWCNT was used as a positive control. Necropsy was performed at days 3 and 60 post-exposure to collect bronchoalveolar lavage fluid (BALF) and lungs.
RESULTS
At day 3 all MWCNTs increased the number of neutrophils in BALF. Chemical purification had a greater effect on pro-inflammatory cytokines (IL-1β, IL-6, CXCL1) in BALF, while thermal purification had a greater effect on pro-fibrotic cytokines (CCL2, OPN, TGF-β1). At day 60, thermally purified, carboxylated MWCNTs had the strongest effect on lymphocyte numbers in BALF. Thermally purified MWCNTs caused the greatest increase in LDH and total protein in BALF. Furthermore, the thermally purified and carboxyl- or amine-functionalized MWCNTs caused the greatest number of granulomatous lesions in the lungs. The physicochemical characteristics mainly associated with increased toxicity of the thermally purified derivatives were decreased surface defects and decreased amorphous content as indicated by Raman spectroscopy.
CONCLUSIONS
These data demonstrate that the purification method is an important determinant of lung toxicity induced by carboxyl- and amine-functionalized MWCNTs.
Identifiants
pubmed: 33243293
doi: 10.1186/s12989-020-00390-y
pii: 10.1186/s12989-020-00390-y
pmc: PMC7690083
doi:
Substances chimiques
Air Pollutants
0
Cytokines
0
Nanotubes, Carbon
0
Transforming Growth Factor beta1
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
60Subventions
Organisme : NIEHS NIH HHS
ID : R01 ES031253
Pays : United States
Organisme : NIEHS NIH HHS
ID : T32 ES007046
Pays : United States
Références
Toxicol Sci. 2006 Jul;92(1):5-22
pubmed: 16484287
Nat Nanotechnol. 2008 Jul;3(7):423-8
pubmed: 18654567
J Nanosci Nanotechnol. 2009 May;9(5):3025-33
pubmed: 19452965
Inhal Toxicol. 2010 Apr;22(5):369-81
pubmed: 20121582
Nanomedicine. 2010 Apr;6(2):245-56
pubmed: 19699321
Proc Natl Acad Sci U S A. 2011 Dec 6;108(49):E1330-8
pubmed: 22084097
Nanotoxicology. 2016 Nov;10(9):1263-75
pubmed: 27323647
PLoS One. 2014 Sep 12;9(9):e106870
pubmed: 25216247
Science. 1998 Jun 12;280(5370):1744-6
pubmed: 9624050
Toxicol Lett. 2006 Aug 1;165(1):88-100
pubmed: 16527436
Toxicol Appl Pharmacol. 2016 May 15;299:125-31
pubmed: 26902652
Int J Environ Res Public Health. 2016 Mar 15;13(3):
pubmed: 26999172
Toxicology. 2010 Mar 10;269(2-3):136-47
pubmed: 19857541
Crit Rev Toxicol. 2010 Apr;40(4):328-46
pubmed: 20128631
PLoS One. 2015 Jun 19;10(6):e0128888
pubmed: 26091108
Toxicol Lett. 2006 Feb 20;161(2):135-42
pubmed: 16229976
Science. 2013 Feb 1;339(6119):535-9
pubmed: 23372006
Am J Respir Cell Mol Biol. 2009 Mar;40(3):349-58
pubmed: 18787175
Nanotoxicology. 2020 Oct;14(8):1058-1081
pubmed: 32813574
Chem Res Toxicol. 2008 Sep;21(9):1698-705
pubmed: 18636756
Inhal Toxicol. 2020 Jan;32(1):24-38
pubmed: 32028803
J Toxicol Environ Health A. 2010;73(5):378-95
pubmed: 20155580
ACS Nano. 2011 Dec 27;5(12):9772-87
pubmed: 22047207
Curr Biol. 2017 Nov 6;27(21):R1173-R1176
pubmed: 29112873
Inflammation. 2010 Aug;33(4):276-80
pubmed: 20174859
Part Fibre Toxicol. 2012 Nov 27;9:46
pubmed: 23181604
Nanotoxicology. 2018 Nov;12(9):975-991
pubmed: 30317900
Inhal Toxicol. 2013 Mar;25(4):199-210
pubmed: 23480196
ACS Nano. 2013 Mar 26;7(3):2352-68
pubmed: 23414138
Anal Biochem. 1981 Oct;117(1):147-57
pubmed: 7316187
Toxicol Appl Pharmacol. 2012 Jun 1;261(2):121-33
pubmed: 22513272
Fibrogenesis Tissue Repair. 2010 Aug 25;3:15
pubmed: 20738867
Nanotoxicology. 2014 May;8(3):317-27
pubmed: 23432020
ACS Nano. 2010 Dec 28;4(12):7241-52
pubmed: 21067152
Part Fibre Toxicol. 2016 Jun 08;13(1):29
pubmed: 27278808
Toxicol Sci. 2013 Jul;134(1):103-10
pubmed: 23570993
Nanomedicine. 2009 Dec;5(4):424-31
pubmed: 19341817
Adv Drug Deliv Rev. 2013 Dec;65(15):2078-86
pubmed: 23899865
Histol Histopathol. 2011 Mar;26(3):357-67
pubmed: 21210349
Chem Res Toxicol. 2012 Jan 13;25(1):74-82
pubmed: 22128750
Nano Res. 2009 Feb 1;2(2):85-120
pubmed: 20174481
Adv Drug Deliv Rev. 2013 Dec;65(15):2063-9
pubmed: 23928473
Inhal Toxicol. 2019 Apr;31(5):192-202
pubmed: 31345048
Angew Chem Int Ed Engl. 2013 Feb 18;52(8):2274-8
pubmed: 23319294
J Toxicol Environ Health A. 2004 Jan 9;67(1):87-107
pubmed: 14668113
Mutat Res. 2017 Nov;823:28-44
pubmed: 28985945
Phys Chem Chem Phys. 2016 May 14;18(18):12784-96
pubmed: 27104221
Am J Respir Cell Mol Biol. 2015 Nov;53(5):625-36
pubmed: 25807359
Environ Health Perspect. 2013 Jun;121(6):676-82
pubmed: 23649427
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2018 May;10(3):e1498
pubmed: 28984415
Toxicol Lett. 2006 Jan 5;160(2):121-6
pubmed: 16125885
PLoS One. 2016 Mar 01;11(3):e0150628
pubmed: 26930275
Curr Biol. 2017 Nov 6;27(21):3302-3314.e6
pubmed: 29112861
Toxicol Appl Pharmacol. 2009 Sep 15;239(3):224-32
pubmed: 19481103
Part Fibre Toxicol. 2014 Jan 09;11:3
pubmed: 24405760
Toxicol In Vitro. 2017 Aug;42:292-298
pubmed: 28483489
Part Fibre Toxicol. 2014 Nov 20;11:59
pubmed: 25410479
Anal Bioanal Chem. 2010 Feb;396(3):1003-14
pubmed: 20052581
Toxicology. 2009 Mar 29;257(3):161-71
pubmed: 19150385
Inhal Toxicol. 2012 Dec;24(14):995-1008
pubmed: 23216160
Toxicol In Vitro. 2015 Oct;29(7):1513-28
pubmed: 26086123
Int J Toxicol. 2018 Jul/Aug;37(4):276-284
pubmed: 29916280
Part Fibre Toxicol. 2013 Jul 30;10:33
pubmed: 23895460
Ann Occup Hyg. 2016 Apr;60(3):305-17
pubmed: 26613611
Ann Occup Hyg. 2015 Jul;59(6):705-23
pubmed: 25851309
Inhal Toxicol. 2008 Jun;20(8):741-9
pubmed: 18569096
Toxicol Sci. 2009 Dec;112(2):468-81
pubmed: 19584127