Significant changes of CD4, FOXP3, CD25, and IL6 expression level in Iranian COVID-19 patients.

COVID-19 Inflammatory responses Interleukin 6 SARS-CoV-2 Treg

Journal

Gastroenterology and hepatology from bed to bench
ISSN: 2008-2258
Titre abrégé: Gastroenterol Hepatol Bed Bench
Pays: Iran
ID NLM: 101525875

Informations de publication

Date de publication:
2020
Historique:
entrez: 27 11 2020
pubmed: 28 11 2020
medline: 28 11 2020
Statut: ppublish

Résumé

Evaluating the expression level of CD4 COVID-19 is an emerging disease with worldwide distribution. However, there is a little data about the correlation between the disease and the host immune responses. Whole blood samples of 30 COVID-19 patients and eight healthy people were collected during March to June 2020. Total RNA was extracted from the samples, cDNA synthesis was performed, and the expression level of targeted genes was evaluated using quantitative real-time PCR. The expression level of CD4, CD25, and Foxp3 was significantly downregulated 5-, 2-, and 3-fold, respectively, among COVID-19 patients in comparison to healthy controls ( Our findings indicated the expression profile analysis of CD4

Sections du résumé

AIM OBJECTIVE
Evaluating the expression level of CD4
BACKGROUND BACKGROUND
COVID-19 is an emerging disease with worldwide distribution. However, there is a little data about the correlation between the disease and the host immune responses.
METHODS METHODS
Whole blood samples of 30 COVID-19 patients and eight healthy people were collected during March to June 2020. Total RNA was extracted from the samples, cDNA synthesis was performed, and the expression level of targeted genes was evaluated using quantitative real-time PCR.
RESULTS RESULTS
The expression level of CD4, CD25, and Foxp3 was significantly downregulated 5-, 2-, and 3-fold, respectively, among COVID-19 patients in comparison to healthy controls (
CONCLUSION CONCLUSIONS
Our findings indicated the expression profile analysis of CD4

Identifiants

pubmed: 33244382
pii: GHFBB-13-388
pmc: PMC7682958

Types de publication

Journal Article

Langues

eng

Pagination

388-392

Informations de copyright

©2020 RIGLD, Research Institute for Gastroenterology and Liver Diseases.

Références

Nature. 2020 Mar;579(7798):270-273
pubmed: 32015507
Blood. 2014 Jul 10;124(2):188-95
pubmed: 24876563
J Infect Dis. 2020 May 11;221(11):1762-1769
pubmed: 32227123
Lancet Respir Med. 2020 Apr;8(4):420-422
pubmed: 32085846
Clin Infect Dis. 2020 Jul 28;71(15):762-768
pubmed: 32161940
J Med Virol. 2006 Apr;78(4):417-24
pubmed: 16482545
J Allergy Clin Immunol. 2020 Jul;146(1):128-136.e4
pubmed: 32425269
J Autoimmun. 2020 Jul;111:102452
pubmed: 32291137
J Leukoc Biol. 2014 Jul;96(1):39-48
pubmed: 24744433
Rev Med Virol. 2020 Nov;30(6):1-9
pubmed: 32845568
J Cell Physiol. 2020 Sep 14;:
pubmed: 32926425
Zhonghua Bing Li Xue Za Zhi. 2020 May 8;49(5):411-417
pubmed: 32172546
N Engl J Med. 2020 Feb 20;382(8):727-733
pubmed: 31978945
FEMS Immunol Med Microbiol. 2012 Apr;64(3):413-24
pubmed: 22211712
Front Immunol. 2020 Jun 16;11:1446
pubmed: 32612617
J Med Virol. 2020 Nov;92(11):2283-2285
pubmed: 32343429
Autoimmun Rev. 2020 Jun;19(6):102537
pubmed: 32251717

Auteurs

Seyed Reza Mohebbi (SR)

Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Kaveh Baghaei (K)

Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Mohammad Rostami-Nejad (M)

Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Ehsan Nazemalhosseini Mojarad (E)

Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Hamed Mirjalali (H)

Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Abbas Yadegar (A)

Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Nastaran Asri (N)

Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Shahrokh Abdoulahi (S)

Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Hamid Assadzadeh Aghdaei (H)

Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Classifications MeSH