Prospective evaluation of two specific IgG immunoassays (HemosIL
Adult
Aged
Aged, 80 and over
Anticoagulants
/ adverse effects
Bayes Theorem
Enzyme-Linked Immunosorbent Assay
/ methods
Female
Heparin
/ adverse effects
Humans
Immunoassay
/ methods
Immunoglobulin G
/ blood
Luminescent Measurements
/ methods
Male
Middle Aged
Prospective Studies
Sensitivity and Specificity
Thrombocytopenia
/ blood
Young Adult
HIT
clinical probability
diagnosis
post-test probability
rapid immuno-assay
Journal
International journal of laboratory hematology
ISSN: 1751-553X
Titre abrégé: Int J Lab Hematol
Pays: England
ID NLM: 101300213
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
revised:
30
09
2020
received:
13
08
2020
accepted:
21
10
2020
pubmed:
28
11
2020
medline:
16
6
2021
entrez:
27
11
2020
Statut:
ppublish
Résumé
The accurate diagnosis of heparin-induced thrombocytopenia (HIT) is essential to ensure adequate treatment and prevent complications. First step diagnosis test are immunoassays including enzyme-linked immunosorbent assays (ELISAs) and rapid immunoassays. Using a Bayesian approach, we prospectively evaluated the performance of the IgG PF4/polyvinylsulfonate ELISA and a chemiluminescent immunoassay (CLIA), which are specific for IgG and use the same antigenic target to detect HIT antibodies. One hundred and eighty-four 184 consecutive patients with an intermediate (n = 159) or high (n = 25) clinical pretest probability of HIT based on the 4Ts score or platelet pattern were included. Both immunoassays (IAs) were performed on all 184 samples, and definite HIT was confirmed with a positive serotonin release assay in 29 patients (12.7%). The sensitivity (Ss) and negative predictive value (NPV) of ELISA were excellent (100%) allowing HIT to be excluded with good confidence when the test was negative. In addition, the Ss and NPV of the CLIA equalled 93.1% and 98.6%, respectively, as it was negative in two definite HIT. When the CLIA was negative, the post-test probability of HIT was 0.7% in case of intermediate risk. Although there was excellent agreement between CLIA and ELISA results, the quantitative values provided by the two IAs were not correlated. AcuStar HIT
Substances chimiques
Anticoagulants
0
Immunoglobulin G
0
Heparin
9005-49-6
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
468-476Subventions
Organisme : Institut pour la Recherche sur la Thrombose et l'Hémostase (IRTH)
Informations de copyright
© 2020 John Wiley & Sons Ltd.
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