Analysis of inositol phosphate metabolism by capillary electrophoresis electrospray ionization mass spectrometry.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
27 11 2020
27 11 2020
Historique:
received:
14
08
2020
accepted:
06
11
2020
entrez:
28
11
2020
pubmed:
29
11
2020
medline:
15
12
2020
Statut:
epublish
Résumé
The analysis of myo-inositol phosphates (InsPs) and myo-inositol pyrophosphates (PP-InsPs) is a daunting challenge due to the large number of possible isomers, the absence of a chromophore, the high charge density, the low abundance, and the instability of the esters and anhydrides. Given their importance in biology, an analytical approach to follow and understand this complex signaling hub is desirable. Here, capillary electrophoresis (CE) coupled to electrospray ionization mass spectrometry (ESI-MS) is implemented to analyze complex mixtures of InsPs and PP-InsPs with high sensitivity. Stable isotope labeled (SIL) internal standards allow for matrix-independent quantitative assignment. The method is validated in wild-type and knockout mammalian cell lines and in model organisms. SIL-CE-ESI-MS enables the accurate monitoring of InsPs and PP-InsPs arising from compartmentalized cellular synthesis pathways, by feeding cells with either [
Identifiants
pubmed: 33247133
doi: 10.1038/s41467-020-19928-x
pii: 10.1038/s41467-020-19928-x
pmc: PMC7695695
doi:
Substances chimiques
Inositol Phosphates
0
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6035Subventions
Organisme : Medical Research Council
ID : MR/T028904/1
Pays : United Kingdom
Références
J Cell Physiol. 2018 Mar;233(3):1897-1912
pubmed: 28542902
Plant Cell. 2015 Apr;27(4):1082-97
pubmed: 25901085
Angew Chem Int Ed Engl. 2014 Sep 1;53(36):9508-11
pubmed: 25044992
ACS Chem Biol. 2019 Oct 18;14(10):2127-2133
pubmed: 31525024
FEBS Lett. 2000 Feb 18;468(1):28-32
pubmed: 10683435
Bio Protoc. 2018 Aug 5;8(15):
pubmed: 30148188
Science. 2007 Nov 23;318(5854):1299-302
pubmed: 18033884
Cell Signal. 2001 Mar;13(3):151-8
pubmed: 11282453
J Med Chem. 2019 Feb 14;62(3):1443-1454
pubmed: 30624931
Mol Plant. 2019 Nov 4;12(11):1463-1473
pubmed: 31419530
Biochem J. 2013 Jun 15;452(3):369-79
pubmed: 23725456
Org Biomol Chem. 2016 Jun 15;14(24):5559-62
pubmed: 26923707
Biochem Soc Symp. 2007;(74):211-21
pubmed: 17233592
Mol Cell. 2018 Jun 7;70(5):936-948.e7
pubmed: 29883610
Top Curr Chem (Cham). 2017 Feb;375(1):14
pubmed: 28101851
J Biol Chem. 2009 Nov 27;284(48):33614-22
pubmed: 19797057
Science. 2011 Nov 11;334(6057):802-5
pubmed: 22076377
J Biol Chem. 1993 Feb 25;268(6):3850-6
pubmed: 8382679
PLoS One. 2014 Jan 09;9(1):e85533
pubmed: 24416420
Biochemistry. 2019 Sep 24;58(38):3927-3932
pubmed: 31461621
Nature. 1983 Nov 3-9;306(5938):67-9
pubmed: 6605482
Biochem J. 1996 May 1;315 ( Pt 3):715-20
pubmed: 8645148
J Biol Chem. 2004 May 21;279(21):21759-65
pubmed: 15024000
Biochem J. 1997 Feb 15;322 ( Pt 1):31-3
pubmed: 9078239
PLoS One. 2016 Oct 27;11(10):e0165286
pubmed: 27788189
Nat Rev Mol Cell Biol. 2001 May;2(5):327-38
pubmed: 11331907
Chem Sci. 2019 Apr 23;10(20):5267-5274
pubmed: 31191882
Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):11968-11973
pubmed: 29078269
Science. 2016 May 20;352(6288):986-90
pubmed: 27080106
Proc Natl Acad Sci U S A. 2019 Dec 3;116(49):24551-24561
pubmed: 31754032
Biochem J. 1983 May 15;212(2):473-82
pubmed: 6309146
Rapid Commun Mass Spectrom. 2009 Mar;23(5):705-12
pubmed: 19191261
Bio Protoc. 2016 Nov 5;6(21):
pubmed: 28573164
J Biol Chem. 2019 Jul 26;294(30):11597-11608
pubmed: 31186349
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Sep 15;879(26):2759-63
pubmed: 21856255
Adv Biol Regul. 2015 Jan;57:203-16
pubmed: 25453220
Plant J. 2014 Nov;80(4):642-53
pubmed: 25231822
Open Biol. 2015 Mar;5(3):150014
pubmed: 25808508
Biochem J. 2020 Jul 31;477(14):2621-2638
pubmed: 32706850
PLoS One. 2009;4(5):e5580
pubmed: 19440344
J Chromatogr A. 2018 Oct 26;1573:87-97
pubmed: 30220429
Angew Chem Int Ed Engl. 2013 Jul 1;52(27):6912-6
pubmed: 23712702
Biochem J. 1988 Sep 1;254(2):585-91
pubmed: 3178774
Nat Protoc. 2006;1(5):2416-22
pubmed: 17406485