Reversion mutation of cDNA CA8-204 minigene construct produces a truncated functional peptide that regulates calcium release in vitro and produces profound analgesia in vivo.


Journal

Mammalian genome : official journal of the International Mammalian Genome Society
ISSN: 1432-1777
Titre abrégé: Mamm Genome
Pays: United States
ID NLM: 9100916

Informations de publication

Date de publication:
12 2020
Historique:
received: 01 07 2020
accepted: 18 09 2020
pubmed: 29 11 2020
medline: 21 8 2021
entrez: 28 11 2020
Statut: ppublish

Résumé

Intracellular calcium is critical in orchestrating neuronal excitability and analgesia. Carbonic anhydrase-8 (CA8) regulates intracellular calcium signaling through allosteric inhibition of neuronal inositol trisphosphate receptor 1 (ITPR1) to produce profound analgesia. Recently, we reported the "G" allele at rs6471859 represents cis-eQTL regulating alternative splicing of a 1697 bp transcript (CA8-204

Identifiants

pubmed: 33247772
doi: 10.1007/s00335-020-09848-y
pii: 10.1007/s00335-020-09848-y
doi:

Substances chimiques

Biomarkers, Tumor 0
DNA, Complementary 0
Peptides 0
Adenosine Triphosphate 8L70Q75FXE
CA8 protein, human EC 4.2.1.1
Calcium SY7Q814VUP

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

287-294

Subventions

Organisme : NINDS NIH HHS
ID : R21 NS105880
Pays : United States

Auteurs

Udita Upadhyay (U)

Department of Anesthesiology, Perioperative Medicine and Pain Management, University of Miami Miller School of Medicine, Miami, FL, USA.

Gerald Z Zhuang (GZ)

Department of Anesthesiology, Perioperative Medicine and Pain Management, University of Miami Miller School of Medicine, Miami, FL, USA.

Luda Diatchenko (L)

Department of Anesthesiology, Alan Edwards Centre for Research on Pain, McGill University, Montreal, QC, Canada.

Marc Parisien (M)

Department of Anesthesiology, Alan Edwards Centre for Research on Pain, McGill University, Montreal, QC, Canada.

Yuan Kang (Y)

Department of Anesthesiology, Perioperative Medicine and Pain Management, University of Miami Miller School of Medicine, Miami, FL, USA.

Konstantinos D Sarantopoulos (KD)

Department of Anesthesiology, Perioperative Medicine and Pain Management, University of Miami Miller School of Medicine, Miami, FL, USA.
Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.

Eden R Martin (ER)

John T. MacDonald Foundation Department of Human Genetics, University of Miami Miller School of Medicine, Miami, FL, USA.
John P. Hussman Institute for Human Genomics, University of Miami Miller School of Medicine, Miami, FL, USA.

Shad B Smith (SB)

Department of Anesthesiology, Center for Translational Pain Medicine, Duke University School of Medicine, Durham, NC, USA.

William Maixner (W)

Department of Anesthesiology, Center for Translational Pain Medicine, Duke University School of Medicine, Durham, NC, USA.

Roy C Levitt (RC)

Department of Anesthesiology, Perioperative Medicine and Pain Management, University of Miami Miller School of Medicine, Miami, FL, USA. rlevitt@med.miami.edu.
Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA. rlevitt@med.miami.edu.
John T. MacDonald Foundation Department of Human Genetics, University of Miami Miller School of Medicine, Miami, FL, USA. rlevitt@med.miami.edu.
John P. Hussman Institute for Human Genomics, University of Miami Miller School of Medicine, Miami, FL, USA. rlevitt@med.miami.edu.
University of Miami Miller School of Medicine, Rosenstiel Medical Sciences Building - Room 8010A (R-371), Miami, FL, 33136, USA. rlevitt@med.miami.edu.

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Classifications MeSH