Methanogen Abundance Thresholds Capable of Differentiating In Vitro Methane Production in Human Stool Samples.
Amplicon sequencing
Gut microbiota
Methane
Methanobrevibacter
Methanogens
Molecular thresholds
Quantitative PCR
mcrA gene
Journal
Digestive diseases and sciences
ISSN: 1573-2568
Titre abrégé: Dig Dis Sci
Pays: United States
ID NLM: 7902782
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
received:
03
09
2020
accepted:
15
11
2020
pubmed:
29
11
2020
medline:
30
11
2021
entrez:
28
11
2020
Statut:
ppublish
Résumé
Intestinal methane (CH In this study, taxonomic and functional gene analyses and in vitro CH We performed a cross-sectional study involving full stool samples collected from 33 healthy individuals. In vitro CH Methanobrevibacter was found to be the most abundant methane producer and its relative abundance provides a clear distinction between CH Given the decreased time-burden placed on patients, a qPCR-based test on a fecal sample can become a valuable tool in clinical assessment of CH
Sections du résumé
BACKGROUND
Intestinal methane (CH
AIMS
In this study, taxonomic and functional gene analyses and in vitro CH
METHODS
We performed a cross-sectional study involving full stool samples collected from 33 healthy individuals. In vitro CH
RESULTS
Methanobrevibacter was found to be the most abundant methane producer and its relative abundance provides a clear distinction between CH
CONCLUSION
Given the decreased time-burden placed on patients, a qPCR-based test on a fecal sample can become a valuable tool in clinical assessment of CH
Identifiants
pubmed: 33247793
doi: 10.1007/s10620-020-06721-5
pii: 10.1007/s10620-020-06721-5
doi:
Substances chimiques
DNA, Archaeal
0
DNA, Bacterial
0
Methane
OP0UW79H66
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3822-3830Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020. Springer Science+Business Media, LLC, part of Springer Nature.
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