Salivary Oral Microbiome of Children With Juvenile Idiopathic Arthritis: A Norwegian Cross-Sectional Study.

Adolescent Arthritis, Juvenile Case-Control Studies Child Cross-Sectional Studies Gemella Humans Microbiota RNA, Ribosomal, 16S / genetics
16S rRNA juvenile idiopathic arthritis next generation sequencing (NGS) oral health salivary microbiome

Journal

Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359

Informations de publication

Date de publication:
2020
Historique:
received: 02 09 2020
accepted: 09 10 2020
entrez: 30 11 2020
pubmed: 1 12 2020
medline: 22 6 2021
Statut: epublish

Résumé

The oral microbiota has been connected to the pathogenesis of rheumatoid arthritis through activation of mucosal immunity. The objective of this study was to characterize the salivary oral microbiome associated with juvenile idiopathic arthritis (JIA), and correlate it with the disease activity including gingival inflammation. Fifty-nine patients with JIA (mean age, 12.6 ± 2.7 years) and 34 healthy controls (HC; mean age 12.3 ± 3.0 years) were consecutively recruited in this Norwegian cross-sectional study. Information about demographics, disease activity, medication history, frequency of tooth brushing and a modified version of the gingival bleeding index (GBI) and the simplified oral hygiene index (OHI-S) was obtained. Microbiome profiling of saliva samples was performed by sequencing of the V1-V3 region of the 16S rRNA gene, coupled with a species-level taxonomy assignment algorithm; QIIME, LEfSe and R-package for Spearman correlation matrix were used for downstream analysis. There were no significant differences between JIA and HC in alpha- and beta-diversity. However, differential abundance analysis revealed several taxa to be associated with JIA: In this exploratory study of salivary oral microbiome we found similar alpha- and beta-diversity among children with JIA and healthy. Several taxa associated with chronic inflammation were found to be associated with JIA and disease activity, which warrants further investigation.

Sections du résumé

Background
The oral microbiota has been connected to the pathogenesis of rheumatoid arthritis through activation of mucosal immunity. The objective of this study was to characterize the salivary oral microbiome associated with juvenile idiopathic arthritis (JIA), and correlate it with the disease activity including gingival inflammation.
Methods
Fifty-nine patients with JIA (mean age, 12.6 ± 2.7 years) and 34 healthy controls (HC; mean age 12.3 ± 3.0 years) were consecutively recruited in this Norwegian cross-sectional study. Information about demographics, disease activity, medication history, frequency of tooth brushing and a modified version of the gingival bleeding index (GBI) and the simplified oral hygiene index (OHI-S) was obtained. Microbiome profiling of saliva samples was performed by sequencing of the V1-V3 region of the 16S rRNA gene, coupled with a species-level taxonomy assignment algorithm; QIIME, LEfSe and R-package for Spearman correlation matrix were used for downstream analysis.
Results
There were no significant differences between JIA and HC in alpha- and beta-diversity. However, differential abundance analysis revealed several taxa to be associated with JIA:
Conclusions
In this exploratory study of salivary oral microbiome we found similar alpha- and beta-diversity among children with JIA and healthy. Several taxa associated with chronic inflammation were found to be associated with JIA and disease activity, which warrants further investigation.

Identifiants

DOI: 10.3389/fcimb.2020.602239 PMID: 33251163 PMC: PMC7672027
pubmed: 33251163
doi: 10.3389/fcimb.2020.602239
pmc: PMC7672027
doi:

Substances chimiques

RNA, Ribosomal, 16S 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

602239

Informations de copyright

Copyright © 2020 Frid, Baraniya, Halbig, Rypdal, Songstad, Rosèn, Berstad, Flatø, Alakwaa, Gil, Cetrelli, Chen, Al-Hebshi, Nordal and Al-Haroni.

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Auteurs

Paula Frid (P)

Department of ENT, Division of Oral and Maxillofacial Surgery, University Hospital North Norway, Tromsø, Norway.
Public Dental Service Competence Centre of North Norway, Tromsø, Norway.
Department of Clinical Medicine, UiT the Arctic University of Norway, Tromsø, Norway.

Divyashri Baraniya (D)

Oral Microbiome Laboratory, Kornberg School of Dentistry, Temple University, Philadelphia, PA, United States.

Josefine Halbig (J)

Public Dental Service Competence Centre of North Norway, Tromsø, Norway.
Department of Clinical Dentistry, UiT the Arctic University of Norway, Tromsø, Norway.

Veronika Rypdal (V)

Department of Clinical Medicine, UiT the Arctic University of Norway, Tromsø, Norway.
Department of Pediatrics and Adolescence Medicine, University Hospital of North Norway, Tromsø, Norway.

Nils Thomas Songstad (NT)

Department of Pediatrics and Adolescence Medicine, University Hospital of North Norway, Tromsø, Norway.

Annika Rosèn (A)

Department of Clinical Dentistry, University of Bergen, Bergen, Norway.
Department of Oral and Maxillofacial Surgery, Haukeland University Hospital, Bergen, Norway.

Johanna Rykke Berstad (JR)

Department of ENT, Division of Oral and Maxillofacial Surgery, Oslo University Hospital, Oslo, Norway.

Berit Flatø (B)

Department of Rheumatology and Infectious Diseases, Institute of Clinical Medicine, University of Oslo, Oslo, Norway.
Department of Rheumatology, Oslo University Hospital, Oslo, Norway.

Fadhl Alakwaa (F)

Department of Computational Medicine and Bioinformatics, University Michigan, Ann Arbor, MI, United States.

Elisabeth Grut Gil (EG)

Department of Clinical Dentistry, University of Bergen, Bergen, Norway.

Lena Cetrelli (L)

Center of Oral Health Services and Research (TkMidt), Trondheim, Norway.

Tsute Chen (T)

Department of Microbiology, Forsyth Institute, Cambridge, MA, United States.

Nezar Noor Al-Hebshi (NN)

Oral Microbiome Laboratory, Kornberg School of Dentistry, Temple University, Philadelphia, PA, United States.

Ellen Nordal (E)

Department of Clinical Medicine, UiT the Arctic University of Norway, Tromsø, Norway.
Department of Pediatrics and Adolescence Medicine, University Hospital of North Norway, Tromsø, Norway.

Mohammed Al-Haroni (M)

Department of Clinical Dentistry, UiT the Arctic University of Norway, Tromsø, Norway.

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Classifications MeSH

questionsmedicales.fr › Personnes › Tranches d'âge › Adolescent › Salivary Oral Microbiome of Children With...
questionsmedicales.fr › Maladies du système immunitaire › Maladies auto-immunes › Arthrite juvénile › Salivary Oral Microbiome of Children With...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Caractéristiques des études épidémiologiques › Études épidémiologiques › Études cas-témoins › Salivary Oral Microbiome of Children With...
questionsmedicales.fr › Personnes › Tranches d'âge › Enfant › Salivary Oral Microbiome of Children With...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Caractéristiques des études épidémiologiques › Études épidémiologiques › Études transversales › Salivary Oral Microbiome of Children With...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bacillales › Gemella › Salivary Oral Microbiome of Children With...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Salivary Oral Microbiome of Children With...
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Biote › Microbiote › Salivary Oral Microbiome of Children With...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN ribosomique › ARN ribosomique 16S › Salivary Oral Microbiome of Children With...