The effect of autophagy and mitochondrial fission on Harderian gland is greater than apoptosis in male hamsters during different photoperiods.
Acetylserotonin O-Methyltransferase
/ metabolism
Animals
Apoptosis
/ physiology
Autophagy
/ physiology
Circadian Rhythm
/ physiology
Cricetinae
/ anatomy & histology
Energy Metabolism
/ physiology
Harderian Gland
/ anatomy & histology
Male
Melatonin
/ metabolism
Mitochondrial Dynamics
/ physiology
Mitochondrial Proton-Translocating ATPases
/ metabolism
Photoperiod
Seasons
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
07
06
2020
accepted:
18
10
2020
entrez:
30
11
2020
pubmed:
1
12
2020
medline:
29
12
2020
Statut:
epublish
Résumé
Photoperiod is an important factor of mammalian seasonal rhythm. Here, we studied morphological differences in the Harderian gland (HG), a vital photosensitive organ, in male striped dwarf hamsters (Cricetulus barabensis) under different photoperiods (short photoperiod, SP; moderate photoperiod, MP; long photoperiod, LP), and investigated the underlying molecular mechanisms related to these morphological differences. Results showed that carcass weight and HG weight were lower under SP and LP conditions. There was an inverse correlation between blood melatonin levels and photoperiod in the order SP > MP > LP. Protein expression of hydroxyindole-O-methyltransferase (HIOMT), a MT synthesis-related enzyme, was highest in the SP group. Protein expression of bax/bcl2 showed no significant differences, indicating that the level of apoptosis remained stable. Protein expression of LC3II/LC3I was higher in the SP group than that in the MP group. Furthermore, comparison of changes in the HG ultrastructure demonstrated autolysosome formation in the LP, suggesting the lowest autophagy level in under MP. Furthermore, the protein expression levels of ATP synthase and mitochondrial fission factor were highest in the MP group, whereas citrate synthase, dynamin-related protein1, and fission1 remained unchanged in the three groups. The change trends of ATP synthase and citrate synthase activity were similar to that of protein expression among the three groups. In summary, the up-regulation of autophagy under SP and LP may be a primary factor leading to loss of HG weight and reduced mitochondrial energy supply capacity.
Identifiants
pubmed: 33253255
doi: 10.1371/journal.pone.0241561
pii: PONE-D-20-17311
pmc: PMC7704011
doi:
Substances chimiques
Acetylserotonin O-Methyltransferase
EC 2.1.1.4
Mitochondrial Proton-Translocating ATPases
EC 3.6.3.-
Melatonin
JL5DK93RCL
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Retracted Publication
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0241561Commentaires et corrections
Type : RetractionIn
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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