Hydrocarbon-Stapled Peptide Based-Nanoparticles for siRNA Delivery.
CPP
Stapled peptides
gene silencing
siRNA
Journal
Nanomaterials (Basel, Switzerland)
ISSN: 2079-4991
Titre abrégé: Nanomaterials (Basel)
Pays: Switzerland
ID NLM: 101610216
Informations de publication
Date de publication:
25 Nov 2020
25 Nov 2020
Historique:
received:
16
10
2020
revised:
09
11
2020
accepted:
21
11
2020
entrez:
1
12
2020
pubmed:
2
12
2020
medline:
2
12
2020
Statut:
epublish
Résumé
Small interfering RNAs (siRNAs) are promising molecules for developing new therapies based on gene silencing; however, their delivery into cells remains an issue. In this study, we took advantage of stapled peptide technology that has emerged as a valuable strategy to render natural peptides more structured, resistant to protease degradation and more bioavailable, to develop short carriers for siRNA delivery. From the pool of stapled peptides that we have designed and synthesized, we identified non-toxic vectors that were able to efficiently encapsulate siRNA, transport them into the cell and induce gene silencing. Remarkably, the most efficient stapled peptide (JMV6582), is composed of only eight amino-acids and contains only two cationic charges.
Identifiants
pubmed: 33255624
pii: nano10122334
doi: 10.3390/nano10122334
pmc: PMC7760004
pii:
doi:
Types de publication
Journal Article
Langues
eng
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