Direct Meta-Analyses Reveal Unexpected Microbial Life in the Highly Radioactive Water of an Operating Nuclear Reactor Core.
16S rRNA amplicon sequencing
environmental microbiome
irradiation
metabarcoding
nuclear reactor
proteotyping
Journal
Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893
Informations de publication
Date de publication:
25 Nov 2020
25 Nov 2020
Historique:
received:
21
10
2020
revised:
16
11
2020
accepted:
23
11
2020
entrez:
1
12
2020
pubmed:
2
12
2020
medline:
2
12
2020
Statut:
epublish
Résumé
The pools of nuclear reactor facilities constitute harsh environments for life, bathed with ionizing radiation, filled with demineralized water and containing toxic radioactive elements. The very few studies published to date have explored water pools used to store spent nuclear fuels. Due to access restrictions and strong handling constraints related to the high radioactivity level, nothing is presently known about life in water pools that directly cool nuclear cores. In this work, we investigated the microbial communities in the cooling pool of the French Osiris nuclear reactor using direct meta-omics approaches, namely, DNA metabarcoding and proteotyping based on 16S ribosomal RNA gene sequencing and on peptide analysis, respectively. We identified 25 genera in the highly radioactive core water supply during operation with radionuclide activity higher than 3 × 10
Identifiants
pubmed: 33255667
pii: microorganisms8121857
doi: 10.3390/microorganisms8121857
pmc: PMC7760952
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Nuclear Toxicology Program of the CEA
ID : Infernus
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