Scratch-induced partial skin wounds re-epithelialize by sheets of independently migrating keratinocytes.
Journal
Life science alliance
ISSN: 2575-1077
Titre abrégé: Life Sci Alliance
Pays: United States
ID NLM: 101728869
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
05
05
2020
revised:
17
11
2020
accepted:
18
11
2020
entrez:
1
12
2020
pubmed:
2
12
2020
medline:
15
9
2021
Statut:
epublish
Résumé
Re-epithelialization is a crucial process to reestablish the protective barrier upon wounding of the skin. Although this process is well described for wounds where the complete epidermis and dermis is damaged, little is known about the re-epithelialization strategy in more frequently occurring smaller scratch wounds in which structures such as the hair follicles and sweat glands stay intact. To study this, we established a scratch wound model to follow individual keratinocytes in all epidermal layers in the back skin of mice by intravital microscopy. We discover that keratinocytes adopt a re-epithelialization strategy that enables them to bypass immobile obstacles such as hair follicles. Wound-induced cell loss is replenished by proliferation in a distinct zone away from the wound and this proliferation does not affect overall migration pattern. Whereas suprabasal keratinocytes are rather passive, basal keratinocytes move as a sheet of independently migrating cells into the wound, thereby constantly changing their direct neighboring cells enabling them to bypass intact obstacles. This re-epithelialization strategy results in a fast re-establishment of the protective skin barrier upon wounding.
Identifiants
pubmed: 33257474
pii: 4/1/e202000765
doi: 10.26508/lsa.202000765
pmc: PMC7723264
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2020 Bornes et al.
Références
J Cell Sci. 2011 Apr 1;124(Pt 7):1017-21
pubmed: 21402875
Nat Commun. 2017 Mar 01;8:14684
pubmed: 28248284
Nat Protoc. 2013 Feb;8(2):302-9
pubmed: 23329003
Genesis. 2007 Sep;45(9):593-605
pubmed: 17868096
FASEB J. 2001 Nov;15(13):2377-89
pubmed: 11689463
J Anat. 2007 Dec;211(6):810-8
pubmed: 18005122
Curr Opin Cell Biol. 2018 Oct;54:89-97
pubmed: 29864721
Adv Wound Care (New Rochelle). 2014 Jul 1;3(7):445-464
pubmed: 25032064
Wound Repair Regen. 2005 Sep-Oct;13(5):468-79
pubmed: 16176455
Clin Dermatol. 1984 Jul-Sep;2(3):24-33
pubmed: 6545767
Physiol Rev. 2003 Jul;83(3):835-70
pubmed: 12843410
Nat Mater. 2011 Jun;10(6):469-75
pubmed: 21602808
Nature. 2007 Feb 8;445(7128):661-5
pubmed: 17251932
Development. 2013 Jan 1;140(1):237-46
pubmed: 23175634
Curr Opin Cell Biol. 2015 Oct;36:62-70
pubmed: 26201843
Proc Natl Acad Sci U S A. 2007 Oct 9;104(41):15988-93
pubmed: 17905871
Cell. 2010 Oct 1;143(1):134-44
pubmed: 20887898
Nature. 2008 May 15;453(7193):314-21
pubmed: 18480812
Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10788-93
pubmed: 12960404
Nat Rev Mol Cell Biol. 2009 Jul;10(7):445-57
pubmed: 19546857
Nat Cell Biol. 2019 Jan;21(1):18-24
pubmed: 30602767
J Cell Sci. 2005 Jan 1;118(Pt 1):51-63
pubmed: 15585576
J Cell Commun Signal. 2016 Jun;10(2):103-20
pubmed: 27170326
J Cell Biol. 1971 May 1;49(2):247-63
pubmed: 19866757
J Cell Biol. 2013 Nov 25;203(4):691-709
pubmed: 24385489
Physiol Rev. 2011 Apr;91(2):691-731
pubmed: 21527735
Trends Cell Biol. 2011 Nov;21(11):638-46
pubmed: 21784638
Cold Spring Harb Perspect Biol. 2017 Apr 3;9(4):
pubmed: 28096261
Nat Cell Biol. 2017 Mar 01;19(2):155-163
pubmed: 28248302