Identification and characterization of distinct brown adipocyte subtypes in C57BL/6J mice.
Adaptor Proteins, Signal Transducing
/ genetics
Adipocytes, Brown
/ metabolism
Adipose Tissue, Brown
/ metabolism
Animals
Basic Helix-Loop-Helix Transcription Factors
/ genetics
Cell Differentiation
/ genetics
Cells, Cultured
Gene Expression Regulation
Gene Knockdown Techniques
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Mitochondria
/ metabolism
Nerve Tissue Proteins
/ genetics
RNA-Seq
/ methods
Signal Transduction
/ genetics
Single-Cell Analysis
/ methods
Transcriptome
Tumor Suppressor Proteins
/ genetics
Uncoupling Protein 1
/ deficiency
Journal
Life science alliance
ISSN: 2575-1077
Titre abrégé: Life Sci Alliance
Pays: United States
ID NLM: 101728869
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
30
09
2020
revised:
13
11
2020
accepted:
15
11
2020
entrez:
1
12
2020
pubmed:
2
12
2020
medline:
15
9
2021
Statut:
epublish
Résumé
Brown adipose tissue (BAT) plays an important role in the regulation of body weight and glucose homeostasis. Although increasing evidence supports white adipose tissue heterogeneity, little is known about heterogeneity within murine BAT. Recently, UCP1 high and low expressing brown adipocytes were identified, but a developmental origin of these subtypes has not been studied. To obtain more insights into brown preadipocyte heterogeneity, we use single-cell RNA sequencing of the BAT stromal vascular fraction of C57/BL6 mice and characterize brown preadipocyte and adipocyte clonal cell lines. Statistical analysis of gene expression profiles from brown preadipocyte and adipocyte clones identify markers distinguishing brown adipocyte subtypes. We confirm the presence of distinct brown adipocyte populations in vivo using the markers EIF5, TCF25, and BIN1. We also demonstrate that loss of
Identifiants
pubmed: 33257475
pii: 4/1/e202000924
doi: 10.26508/lsa.202000924
pmc: PMC7723269
pii:
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Basic Helix-Loop-Helix Transcription Factors
0
Bin1 protein, mouse
0
Nerve Tissue Proteins
0
Nulp1 protein, mouse
0
Tumor Suppressor Proteins
0
Ucp1 protein, mouse
0
Uncoupling Protein 1
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2020 Karlina et al.
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