Essential omega-3 fatty acids tune microglial phagocytosis of synaptic elements in the mouse developing brain.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
30 11 2020
Historique:
received: 24 07 2019
accepted: 03 11 2020
entrez: 1 12 2020
pubmed: 2 12 2020
medline: 22 12 2020
Statut: epublish

Résumé

Omega-3 fatty acids (n-3 PUFAs) are essential for the functional maturation of the brain. Westernization of dietary habits in both developed and developing countries is accompanied by a progressive reduction in dietary intake of n-3 PUFAs. Low maternal intake of n-3 PUFAs has been linked to neurodevelopmental diseases in Humans. However, the n-3 PUFAs deficiency-mediated mechanisms affecting the development of the central nervous system are poorly understood. Active microglial engulfment of synapses regulates brain development. Impaired synaptic pruning is associated with several neurodevelopmental disorders. Here, we identify a molecular mechanism for detrimental effects of low maternal n-3 PUFA intake on hippocampal development in mice. Our results show that maternal dietary n-3 PUFA deficiency increases microglia-mediated phagocytosis of synaptic elements in the rodent developing hippocampus, partly through the activation of 12/15-lipoxygenase (LOX)/12-HETE signaling, altering neuronal morphology and affecting cognitive performance of the offspring. These findings provide a mechanistic insight into neurodevelopmental defects caused by maternal n-3 PUFAs dietary deficiency.

Identifiants

pubmed: 33257673
doi: 10.1038/s41467-020-19861-z
pii: 10.1038/s41467-020-19861-z
pmc: PMC7704669
doi:

Substances chimiques

Fatty Acids, Omega-3 0
Lipoxygenase EC 1.13.11.12

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

6133

Subventions

Organisme : NINDS NIH HHS
ID : R01 NS088137
Pays : United States
Organisme : CIHR
Pays : Canada

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Auteurs

C Madore (C)

Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France.
Ann Romney Center for Neurologic Diseases, Department of Neurology, Brigham and Women´s Hospital, Harvard Medical School, Boston, MA, USA.

Q Leyrolle (Q)

Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France.
NeuroDiderot, Inserm, Université de Paris Diderot, F-75019, Paris, France.

L Morel (L)

Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France.

M Rossitto (M)

Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France.

A D Greenhalgh (AD)

Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France.

J C Delpech (JC)

Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France.

M Martinat (M)

Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France.

C Bosch-Bouju (C)

Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France.

J Bourel (J)

Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France.

B Rani (B)

Department of Health Sciences, University of Florence, Florence, Italy.

C Lacabanne (C)

Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France.

A Thomazeau (A)

Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France.

K E Hopperton (KE)

Department of Nutritional Sciences, University of Toronto, Toronto, ON, M5S 3E2, Canada.

S Beccari (S)

Achucarro Basque Center for Neuroscience, University of the Basque Country and Ikerbasque Foundation, 48940, Leioa, Spain.

A Sere (A)

Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France.

A Aubert (A)

Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France.

V De Smedt-Peyrusse (V)

Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France.

C Lecours (C)

Neurosciences Axis, CRCHU de Québec-Université Laval, Québec City, QC, Canada.

K Bisht (K)

Neurosciences Axis, CRCHU de Québec-Université Laval, Québec City, QC, Canada.

L Fourgeaud (L)

Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, 92037, USA.

S Gregoire (S)

Centre des Sciences du Goût et de l'Alimentation, AgroSup Dijon, CNRS, INRAE, Univ. Bourgogne Franche-Comté, F-21000, Dijon, France.

L Bretillon (L)

Centre des Sciences du Goût et de l'Alimentation, AgroSup Dijon, CNRS, INRAE, Univ. Bourgogne Franche-Comté, F-21000, Dijon, France.

N Acar (N)

Centre des Sciences du Goût et de l'Alimentation, AgroSup Dijon, CNRS, INRAE, Univ. Bourgogne Franche-Comté, F-21000, Dijon, France.

N J Grant (NJ)

CNRS UPR3212, Institut des Neurosciences Cellulaires et Intégratives, Strasbourg, France.

J Badaut (J)

CNRS UMR5287, University of Bordeaux, Bordeaux, France.

P Gressens (P)

NeuroDiderot, Inserm, Université de Paris Diderot, F-75019, Paris, France.
Centre for the Developing Brain, Department of Division of Imaging Sciences and Biomedical Engineering, King's College London, King's Health Partners, St. Thomas' Hospital, London, SE1 7EH, UK.

A Sierra (A)

Achucarro Basque Center for Neuroscience, University of the Basque Country and Ikerbasque Foundation, 48940, Leioa, Spain.

O Butovsky (O)

Ann Romney Center for Neurologic Diseases, Department of Neurology, Brigham and Women´s Hospital, Harvard Medical School, Boston, MA, USA.
Evergrande Center for Immunologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

M E Tremblay (ME)

Neurosciences Axis, CRCHU de Québec-Université Laval, Québec City, QC, Canada.

R P Bazinet (RP)

Department of Nutritional Sciences, University of Toronto, Toronto, ON, M5S 3E2, Canada.

C Joffre (C)

Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France.

A Nadjar (A)

Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France. agnes.nadjar@u-bordeaux.fr.

S Layé (S)

Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286, F-33000, Bordeaux, France. sophie.laye@inrae.fr.

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