Human amniotic membrane mesenchymal stem cell-conditioned medium reduces inflammatory factors and fibrosis in ovalbumin-induced asthma in mice.
asthma
conditioned medium
fibrosis
human amniotic membrane
inflammatory factors
mesenchymal stem cell
Journal
Experimental physiology
ISSN: 1469-445X
Titre abrégé: Exp Physiol
Pays: England
ID NLM: 9002940
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
06
07
2020
accepted:
24
11
2020
pubmed:
2
12
2020
medline:
25
2
2022
entrez:
1
12
2020
Statut:
ppublish
Résumé
What is the central question of this study? Is mesenchymal stem cell-conditioned medium capable of improving the pathological alterations of ovalbumin-induced asthma in mice? What is the main finding and its importance? Our study indicated that human amniotic membrane mesenchymal stem cell-conditioned medium is capable of modulating inflammation, fibrosis, oxidative stress and the pathological consequences of ovalbumin-induced allergic asthma in mice. Paracrine factors secreted by mesenchymal stem cells (MSCs) have immunomodulatory, anti-inflammatory and antifibrotic properties, and the conditioned medium (CM) of these cells might have functional capabilities. We examined the effects of human amniotic membrane MSC-CM (hAM-MSC-CM) on ovalbumin (OVA)-induced asthma. Forty male Balb/c mice were randomly divided into the following four groups: control; OVA (sensitized and challenged with OVA); OVA+CM (sensitized and challenged with OVA and treated with hAM-MSC-CM); and OVA+Placebo (sensitized and challenged with OVA and treated with placebo). Forty-eight hours after the last challenge, serum and bronchoalveolar lavage fluid samples were collected and used for evaluation of inflammatory factors and cells, respectively. Lung tissue sections were stained with Haematoxylin and Eosin or Masson's Trichrome to evaluate pathological changes, and oxidative stress was assessed in fresh lung tissues. Treatment with hAM-MSC-CM significantly hindered histopathological changes and fibrosis and reduced the total cell count and the percentage of eosinophils and neutrophils in bronchoalveolar lavage fluid. Furthermore, it reduced serum levels of immunoglobulin E, interleukin-4, transforming growth factor-β and lung malondialdehyde. It also increased serum levels of interferon-γ and interleukin-10, in addition to the enzymatic activity of glutathione peroxidase, catalase and superoxide dismutase in lung tissue in comparison to the OVA and OVA+Placebo groups. This study showed that administration of hAM-MSC-CM can improve pathological conditions, such as inflammation, fibrosis and oxidative stress, in OVA-induced allergic asthma.
Substances chimiques
Culture Media, Conditioned
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
544-554Informations de copyright
© 2020 The Authors. Experimental Physiology © 2020 The Physiological Society.
Références
Aboutaleb, N., Faezi, M., Nasseri Maleki, S., Nazarinia, D., Razavi Tousi, S. M. T., & Hashemirad, N. (2019). Conditioned medium obtained from mesenchymal stem cells attenuates focal cerebral ischemia reperfusion injury through activation of ERK1/ERK2-BDNF signalling pathway. Journal of Chemical Neuroanatomy, 97, 87-98.
Ahmadi, M., Rahbarghazi, R., Aslani, M. R., Shahbazfar, A.-A., Kazemi, M., & Keyhanmanesh, R. (2017). Bone marrow mesenchymal stem cells and their conditioned media could potentially ameliorate ovalbumin-induced asthmatic changes. Biomedicine & Pharmacotherapy, 85, 28-40.
Bhuiyan, S. I., Sayed, M. A., Mustafiz, M. I., Kabir, M. J., & Aktar, J. (2018). Metered dose inhaler (MDI) versus dry powder inhaler (DPI): Patient's compliance variation in asthma medication at rural Bangladesh perspective. Journal of Dental and Medical Sciences, 17, 66-75.
Bloemen, K., Verstraelen, S., Van Den Heuvel, R., Witters, H., Nelissen, I., & Schoeters, G. (2007). The allergic cascade: Review of the most important molecules in the asthmatic lung. Immunology Letters, 113, 6-18.
Chen, Z.-Y., Hu, Y.-Y., Hu, X.-F., & Cheng, L.-X. (2018). The conditioned medium of human mesenchymal stromal cells reduces irradiation-induced damage in cardiac fibroblast cells. Journal of Radiation Research, 59, 555-564.
Cho, Y. S., & Moon, H.-B. (2010). The role of oxidative stress in the pathogenesis of asthma. Allergy, Asthma & Immunology Research, 2, 183-187.
Choi, J. S., Jeong, I. S., Han, J. H., Cheon, S. H., & Kim, S-W. (2019). IL-10-secreting human MSCs generated by TALEN gene editing ameliorate liver fibrosis through enhanced anti-fibrotic activity. Biomaterials Science, 7, 1078-1087.
Chow, L. N., Schreiner, P., Ng, B. Y., Lo, B., Hughes, M. R., Scott, R. W., Gusti, V., Lecour, S., Simonson, E., Barta, I., McNagny, K. M., Crawford, J., Webb, M., Underhill, T. M., & Manisali, I. (2016). Impact of a CXCL12/CXCR4 antagonist in bleomycin (BLM) induced pulmonary fibrosis and carbon tetrachloride (CCl4) induced hepatic fibrosis in mice. PLoS One, 11, e0151765.
Dai, R., Yu, Y., Yan, G., Hou, X., Ni, Y., & Shi, G. (2018). Intratracheal administration of adipose derived mesenchymal stem cells alleviates chronic asthma in a mouse model. BMC Pulmonary Medicine, 18, 131.
Diver, S., Russell, R. J., & Brightling, C. E. (2018). New and emerging drug treatments for severe asthma. Clinical & Experimental Allergy, 48, 241-252.
Erle, D. J., & Sheppard, D. (2014). The cell biology of asthma. The Journal of Cell Biology, 205, 621-631.
Francisco, J. C., Cunha, R. C., Simeoni, R. B., Guarita-Souza, L. C., Ferreira, R. J., Irioda, A. C., Souza, C. M. C. O., Srikanth, G. V. N., Nityanand, S., Chachques, J. C., & de Carvalho, K. A. T. (2013). Amniotic membrane as a potent source of stem cells and a matrix for engineering heart tissue. Journal of Biomedical Science and Engineering, 06, 1178-1185.
Fu, Q., Ohnishi, S., & Sakamoto, N. (2018). Conditioned medium from human amnion-derived mesenchymal stem cells regulates activation of primary hepatic stellate cells. Stem Cells International, 2018, 4898152.
Gong, P., Li, Y., Tan, Y.-P., & Li, H. (2015). Pretreatment with inactivated Bacillus Calmette-Guerin increases CD4+CD25+ regulatory T cell function and decreases functional and structural effects of asthma induction in a rat asthma model. Artificial Organs, 40, 360-367.
Grundy, D. (2015). Principles and standards for reporting animal experiments in The Journal of Physiology and Experimental Physiology. Experimental Physiology, 100, 755-758.
Halwani, R., Al-Muhsen, S., Al-Jahdali, H., & Hamid, Q. (2011). Role of transforming growth factor-β in airway remodeling in asthma. American Journal of Respiratory Cell and Molecular Biology, 44, 127-133.
Hamelmann, E., Tadeda, K., Oshiba, A., & Gelfand, E. W. (1999). Role of IgE in the development of allergic airway inflammation and airway hyperresponsiveness - a murine model. Allergy, 54, 297-305.
Husain, Q., Ahmad, A., & Shameem, M. (2012). Relation of oxidant-antioxidant imbalance with disease progression in patients with asthma. Annals of Thoracic Medicine, 7, 226-232.
Ishmael, F. T. (2011). The inflammatory response in the pathogenesis of asthma. The Journal of the American Osteopathic Association, 111, S11-S17.
Ito, I., Fixman, E. D., Asai, K., Yoshida, M., Gounni, A. S., Martin, J. G., & Hamid, Q. (2009). Platelet-derived growth factor and transforming growth factor-β modulate the expression of matrix metalloproteinases and migratory function of human airway smooth muscle cells. Clinical & Experimental Allergy, 39, 1370-1380.
Janssen-Heininger, Y. M. W., Poynter, M. E., Aesif, S. W., Pantano, C., Ather, J. L., Reynaert, N. L., Ckless, K., Anathy, V., van der Velden, J., Irvin, C. G., & van der Vliet, A. (2009). Nuclear factor κB, airway epithelium, and asthma: Avenues for redox control. Proceedings of the American Thoracic Society, 6, 249-255.
Keyhanmanesh, R., Rahbarghazi, R., & Ahmadi, M. (2018). Systemic transplantation of mesenchymal stem cells modulates endothelial cell adhesion molecules induced by ovalbumin in rat model of asthma. Inflammation, 41, 2236-2245.
Liang, X., Wang, J., Chen, W., Ma, X., Wang, Y., Nagao, N., Weng, W., Huang, J., & Liu, J. (2017). Inhibition of airway remodeling and inflammation by isoforskolin in PDGF-induced rat ASMCs and OVA-induced rat asthma model. Biomedicine & Pharmacotherapy, 95, 275-286.
Lloyd, C. M., & Hessel, E. M. (2010). Functions of T cells in asthma: More than just TH 2 cells. Nature Reviews Immunology, 10, 838-848.
Lloyd, C. M., & Robinson, D. S. (2007). Allergen-induced airway remodelling. European Respiratory Journal, 29, 1020-1032.
Locke, N. R., Royce, S. G., Wainewright, J. S., Samuel, C. S., & Tang, M. L. (2007). Comparison of airway remodeling in acute, subacute, and chronic models of allergic airways disease. American Journal of Respiratory Cell and Molecular Biology, 36, 625-632.
Luo, J., Zhang, L., Zhang, X., Long, Y., Zou, F., Yan, C., & Zou, W. (2019). Protective effects and active ingredients of Salvia miltiorrhiza Bunge extracts on airway responsiveness, inflammation and remodeling in mice with ovalbumin-induced allergic asthma. Phytomedicine, 52, 168-177.
Magatti, M., Caruso, M., De Munari, S., Vertua, E., De, D., Manuelpillai, U., & Parolini, O. (2015). Human amniotic membrane-derived mesenchymal and epithelial cells exert different effects on monocyte-derived dendritic cell differentiation and function. Cell Transplantation, 24, 1733-1752.
Martin, J. G., & Verma, N. (2012). Mechanisms of airway remodeling in asthma. Drug Discovery Today: Disease Mechanisms, 9, e95-e102.
Mihu, C. M., Rus Ciucă, D., Soritau, O., Suşman, S., & Mihu, D. (2009). Isolation and characterization of mesenchymal stem cells from the amniotic membrane. Romanian Journal of Morphology and Embryology, 50, 73-77.
Mohammadian, M., Boskabady, M. H., Kashani, I. R., Jahromi, G. P., Omidi, A., Nejad, A. K., Khamse, S., & Sadeghipour, H. R. (2016). Effect of bone marrow derived mesenchymal stem cells on lung pathology and inflammation in ovalbumin-induced asthma in mouse. Iranian Journal of Basic Medical Sciences, 19, 55-63.
Murdoch, J. R., & Lloyd, C. M. (2010). Chronic inflammation and asthma. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 690, 24-39.
Naiki, Y., Michelsen, K. S., Schröder, N. W. J., Alsabeh, R., Slepenkin, A., Zhang, W., Chen, S., Wei, B., Bulut, Y., Wong, M. H., Peterson, E. M., & Arditi, M. (2005). MyD88 is pivotal for the early inflammatory response and subsequent bacterial clearance and survival in a mouse model of Chlamydia pneumoniae pneumonia. Journal of Biological Chemistry, 280, 29242-29249.
Nakagome, K., & Nagata, M. (2011). Pathogenesis of airway inflammation in bronchial asthma. Auris Nasus Larynx, 38, 555-563.
Navas, A., Magaña-Guerrero, F. S., Domínguez-López, A., Chávez-García, C., Partido, G., Graue-Hernández, E. O., Sánchez-García, F. J., & Garfias, Y. (2018). Anti-inflammatory and anti-fibrotic effects of human amniotic membrane mesenchymal stem cells and their potential in corneal repair. Stem Cells Translational Medicine, 7, 906-917.
Pan, C., Lang, H., Zhang, T., Wang, R., Lin, X., Shi, P., Zhao, F., & Pang, X. (2019). Conditioned medium derived from human amniotic stem cells delays H2O2induced premature senescence in human dermal fibroblasts. International Journal of Molecular Medicine, 44, 1629-1640.
Qu, J., Li, Y., Zhong, W., Gao, P., & Hu, C. (2017). Recent developments in the role of reactive oxygen species in allergic asthma. Journal of Thoracic Disease, 9, E32.
Rehman, A., Amin, F., & Sadeeqa, S. (2018). Prevalence of asthma and its management: A review. JPMA. The Journal of the Pakistan Medical Association, 68, 1823-1827.
Riedel, R., Pérez-Pérez, A., Carmona-Fernández, A., Jaime, M., Casale, R., Dueñas, J. L., Guadix, P., Sánchez-Margalet V., Varone, C. L., & Maymó, J. L. (2019). Human amniotic membrane conditioned medium inhibits proliferation and modulates related microRNAs expression in hepatocarcinoma cells. Scientific Reports, 9, 14193.
Rossi, D., Pianta, S., Magatti, M., Sedlmayr, P., & Parolini, O. (2012). Characterization of the conditioned medium from amniotic membrane cells: Prostaglandins as key effectors of its immunomodulatory activity. PLoS ONE, 7, e46956.
Rydell-Törmänen, K., Risse, P.-A., Kanabar, V., Bagchi, R., Czubryt, M. P., & Johnson, J. R. (2013). Smooth muscle in tissue remodeling and hyper-reactivity: Airways and arteries. Pulmonary Pharmacology & Therapeutics, 26, 13-23.
Sato, C., Yamamoto, Y., Funayama, E., Furukawa, H., Oyama, A., Murao, N., Hosono, H., Kawakubo, K., Sakamoto, N., & Ohnishi, S. (2018). Conditioned medium obtained from amnion-derived mesenchymal stem cell culture prevents activation of keloid fibroblasts. Plastic and Reconstructive Surgery, 141, 390-398.
Silva, L. H. A., Antunes, M. A., Dos Santos, C. C., Weiss, D. J., Cruz, F. F., & Rocco, P. R. M. (2018). Strategies to improve the therapeutic effects of mesenchymal stromal cells in respiratory diseases. Stem Cell Research & Therapy, 9, 45.
Tagaya, E., & Tamaoki, J. (2007). Mechanisms of airway remodeling in asthma. Allergology International, 56, 331-340.
Tousi, S. M. T. R., Amirizadeh, N., Nasirinezhad, F., Nikougoftar, M., Ganjibakhsh, M., & Aboutaleb, N. (2017). A rapid and cost-effective protocol for isolating mesenchymal stem cells from the human amniotic membrane. Galen Medical Journal, 6, 217-225.
Zemmouri, H., Sekiou, O., Ammar, S., El Feki, A., Bouaziz, M., Messarah, M., & Boumendjel, A. (2017). Urtica dioica attenuates ovalbumin-induced inflammation and lipid peroxidation of lung tissues in rat asthma model. Pharmaceutical Biology, 55, 1561-1568.
Zhang, C., Yang, P., Chen, Y., Liu, J., & Yuan, X. (2018). Expression of DACT1 in children with asthma and its regulation mechanism. Experimental and Therapeutic Medicine, 15, 2674-2680.
Zhao, Q., Ren, H., & Han, Z. (2016). Mesenchymal stem cells: Immunomodulatory capability and clinical potential in immune diseases. Journal of Cellular Immunotherapy, 2, 3-20.
Zhou, Y., Zhang, Q., Gao, Y., Tan, M., Zheng, R., Zhao, L., & Zhang, X. (2018). Induced pluripotent stem cell-conditioned medium suppresses pulmonary fibroblast-to-myofibroblast differentiation via the inhibition of TGF-β1/Smad pathway. International Journal of Molecular Medicine, 41, 473-484.