Prosaposin and its receptors GRP37 and GPR37L1 show increased immunoreactivity in the facial nucleus following facial nerve transection.
Animals
Astrocytes
/ metabolism
Facial Nerve
/ metabolism
Facial Nucleus
/ metabolism
Gene Expression Regulation
/ genetics
Humans
Microglia
/ metabolism
Motor Neurons
/ metabolism
Nerve Regeneration
/ genetics
Nerve Tissue Proteins
/ genetics
RNA, Messenger
/ genetics
Rats
Receptors, G-Protein-Coupled
/ genetics
Saposins
/ genetics
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
30
06
2020
accepted:
13
10
2020
entrez:
1
12
2020
pubmed:
2
12
2020
medline:
15
1
2021
Statut:
epublish
Résumé
Neurotrophic factor prosaposin (PS) is a precursor for saposins A, B, C, and D, which are activators for specific sphingolipid hydrolases in lysosomes. Both saposins and PS are widely contained in various tissues. The brain, skeletal muscle, and heart cells predominantly contain unprocessed PS rather than saposins. PS and PS-derived peptides stimulate neuritogenesis and increase choline acetyltransferase activity in neuroblastoma cells and prevent programmed cell death in neurons. We previously detected increases in PS immunoactivity and its mRNA in the rat facial nucleus following facial nerve transection. PS mRNA expression increased not only in facial motoneurons, but also in microglia during facial nerve regeneration. In the present study, we examined the changes in immunoreactivity of the PS receptors GPR37 and GPR37L1 in the rat facial nucleus following facial nerve transection. Following facial nerve transection, many small Iba1- and glial fibrillary acidic protein (GFAP)-positive cells with strong GPR37L1 immunoreactivity, including microglia and astrocytes, were observed predominately on the operated side. These results indicate that GPR37 mainly works in neurons, whereas GPR37L1 is predominant in microglia or astrocytes, and suggest that increased PS in damaged neurons stimulates microglia or astrocytes via PS receptor GPR37L1 to produce neurotrophic factors for neuronal recovery.
Identifiants
pubmed: 33259479
doi: 10.1371/journal.pone.0241315
pii: PONE-D-20-17075
pmc: PMC7707515
doi:
Substances chimiques
GPR37L1 protein, human
0
Gpr37 protein, rat
0
Nerve Tissue Proteins
0
Psap protein, rat
0
RNA, Messenger
0
Receptors, G-Protein-Coupled
0
Saposins
0
Banques de données
figshare
['10.6084/m9.figshare.13008446', '10.6084/m9.figshare.13006646', '10.6084/m9.figshare.13127459', '10.6084/m9.figshare.13127561', '10.6084/m9.figshare.13127624', '10.6084/m9.figshare.13127654', '10.6084/m9.figshare.13127708', '10.6084/m9.figshare.13127741']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0241315Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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