Detection of SARS-CoV-2 within the healthcare environment: a multi-centre study conducted during the first wave of the COVID-19 outbreak in England.
Aerosols
COVID-19
/ diagnosis
Cross Infection
/ prevention & control
Disease Outbreaks
/ prevention & control
Disinfection
/ methods
England
/ epidemiology
Female
Fomites
/ statistics & numerical data
Health Facilities
/ statistics & numerical data
Health Personnel
/ education
Hospitals
/ statistics & numerical data
Humans
Infection Control
/ methods
Male
Personal Protective Equipment
/ standards
RNA, Viral
/ genetics
Reverse Transcriptase Polymerase Chain Reaction
/ methods
SARS-CoV-2
/ genetics
Air
Environmental contamination
Environmental surfaces
Hospital
Infection control
SARS-CoV-2
Journal
The Journal of hospital infection
ISSN: 1532-2939
Titre abrégé: J Hosp Infect
Pays: England
ID NLM: 8007166
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
01
10
2020
revised:
25
11
2020
accepted:
25
11
2020
pubmed:
2
12
2020
medline:
7
2
2021
entrez:
1
12
2020
Statut:
ppublish
Résumé
Understanding how severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is spread within the hospital setting is essential in order to protect staff, implement effective infection control measures, and prevent nosocomial transmission. The presence of SARS-CoV-2 in the air and on environmental surfaces around hospitalized patients, with and without respiratory symptoms, was investigated. Environmental sampling was undertaken within eight hospitals in England during the first wave of the coronavirus disease 2019 outbreak. Samples were analysed using reverse transcription polymerase chain reaction (PCR) and virus isolation assays. SARS-CoV-2 RNA was detected on 30 (8.9%) of 336 environmental surfaces. Cycle threshold values ranged from 28.8 to 39.1, equating to 2.2 x 10 Effective cleaning can reduce the risk of fomite (contact) transmission, but some surface types may facilitate the survival, persistence and/or dispersal of SARS-CoV-2. The presence of low or undetectable concentrations of viral RNA in the air supports current guidance on the use of specific personal protective equipment for aerosol-generating and non-aerosol-generating procedures.
Sections du résumé
BACKGROUND
BACKGROUND
Understanding how severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is spread within the hospital setting is essential in order to protect staff, implement effective infection control measures, and prevent nosocomial transmission.
METHODS
METHODS
The presence of SARS-CoV-2 in the air and on environmental surfaces around hospitalized patients, with and without respiratory symptoms, was investigated. Environmental sampling was undertaken within eight hospitals in England during the first wave of the coronavirus disease 2019 outbreak. Samples were analysed using reverse transcription polymerase chain reaction (PCR) and virus isolation assays.
FINDINGS
RESULTS
SARS-CoV-2 RNA was detected on 30 (8.9%) of 336 environmental surfaces. Cycle threshold values ranged from 28.8 to 39.1, equating to 2.2 x 10
CONCLUSIONS
CONCLUSIONS
Effective cleaning can reduce the risk of fomite (contact) transmission, but some surface types may facilitate the survival, persistence and/or dispersal of SARS-CoV-2. The presence of low or undetectable concentrations of viral RNA in the air supports current guidance on the use of specific personal protective equipment for aerosol-generating and non-aerosol-generating procedures.
Identifiants
pubmed: 33259882
pii: S0195-6701(20)30548-X
doi: 10.1016/j.jhin.2020.11.024
pmc: PMC7831847
pii:
doi:
Substances chimiques
Aerosols
0
RNA, Viral
0
Types de publication
Journal Article
Multicenter Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
189-196Subventions
Organisme : Medical Research Council
ID : MC_PC_19064
Pays : United Kingdom
Commentaires et corrections
Type : CommentIn
Type : CommentIn
Informations de copyright
Crown Copyright © 2020. Published by Elsevier Ltd. All rights reserved.