Reduced Biliverdin Reductase-A Expression in Visceral Adipose Tissue is Associated with Adipocyte Dysfunction and NAFLD in Human Obesity.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
29 Nov 2020
Historique:
received: 07 11 2020
revised: 24 11 2020
accepted: 27 11 2020
entrez: 2 12 2020
pubmed: 3 12 2020
medline: 6 3 2021
Statut: epublish

Résumé

Biliverdin reductase A (BVR-A) is an enzyme involved in the regulation of insulin signalling. Knockout (KO) mice for hepatic BVR-A, on a high-fat diet, develop more severe glucose impairment and hepato-steatosis than the wild type, whereas loss of adipocyte BVR-A is associated with increased visceral adipose tissue (VAT) inflammation and adipocyte size. However, BVR-A expression in human VAT has not been investigated. We evaluated BVR-A mRNA expression levels by real-time PCR in the intra-operative omental biopsy of 38 obese subjects and investigated the association with metabolic impairment, VAT dysfunction, and biopsy-proven non-alcoholic fatty liver disease (NAFLD). Individuals with lower VAT BVR-A mRNA levels had significantly greater VAT IL-8 and Caspase 3 expression than those with higher BVR-A. Lower VAT BVR-A mRNA levels were associated with an increased adipocytes' size. An association between lower VAT BVR-A expression and higher plasma gamma-glutamyl transpeptidase was also observed. Reduced VAT BVR-A was associated with NAFLD with an odds ratio of 1.38 (95% confidence interval: 1.02-1.9; χ

Identifiants

pubmed: 33260451
pii: ijms21239091
doi: 10.3390/ijms21239091
pmc: PMC7730815
pii:
doi:

Substances chimiques

Cytokines 0
RNA, Messenger 0
Oxidoreductases Acting on CH-CH Group Donors EC 1.3.-
biliverdin reductase EC 1.3.1.24
Caspase 3 EC 3.4.22.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Valentina Ceccarelli (V)

Department of Experimental Medicine, Sapienza University of Rome, 00161 Rome, Italy.

Ilaria Barchetta (I)

Department of Experimental Medicine, Sapienza University of Rome, 00161 Rome, Italy.

Flavia Agata Cimini (FA)

Department of Experimental Medicine, Sapienza University of Rome, 00161 Rome, Italy.

Laura Bertoccini (L)

Department of Experimental Medicine, Sapienza University of Rome, 00161 Rome, Italy.

Caterina Chiappetta (C)

Department of Medical-Surgical Sciences and Bio-Technologies, Sapienza University of Rome, 04100 Latina, Italy.

Danila Capoccia (D)

Department of Medical-Surgical Sciences and Bio-Technologies, Sapienza University of Rome, 04100 Latina, Italy.

Raffaella Carletti (R)

Department of Medical-Surgical Sciences and Bio-Technologies, Sapienza University of Rome, 04100 Latina, Italy.

Claudio Di Cristofano (C)

Department of Medical-Surgical Sciences and Bio-Technologies, Sapienza University of Rome, 04100 Latina, Italy.

Gianfranco Silecchia (G)

Department of Medical-Surgical Sciences and Bio-Technologies, Sapienza University of Rome, 04100 Latina, Italy.

Mario Fontana (M)

Department of Biochemical Sciences "A. Rossi-Fanelli" Sapienza University of Rome, 00185 Rome, Italy.

Frida Leonetti (F)

Department of Medical-Surgical Sciences and Bio-Technologies, Sapienza University of Rome, 04100 Latina, Italy.

Andrea Lenzi (A)

Department of Experimental Medicine, Sapienza University of Rome, 00161 Rome, Italy.

Marco Giorgio Baroni (MG)

Department of Clinical Medicine, Public Health, Life and Environmental Sciences (MeSVA), University of L'Aquila, 67100 Coppito, Italy.
Neuroendocrinology and Metabolic Diseases, IRCCS Neuromed, 86077 Pozzilli, Italy.

Eugenio Barone (E)

Department of Biochemical Sciences "A. Rossi-Fanelli" Sapienza University of Rome, 00185 Rome, Italy.

Maria Gisella Cavallo (MG)

Department of Experimental Medicine, Sapienza University of Rome, 00161 Rome, Italy.

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Classifications MeSH