Potential caveats of putative microglia-specific markers for assessment of age-related cerebrovascular neuroinflammation.
Aging
Brain infiltrating myeloid cells
CD45
Cerebral amyloid angiopathy
Microglia
Neuroinflammation
P2RY12
Stroke
Tmem119
Journal
Journal of neuroinflammation
ISSN: 1742-2094
Titre abrégé: J Neuroinflammation
Pays: England
ID NLM: 101222974
Informations de publication
Date de publication:
01 Dec 2020
01 Dec 2020
Historique:
received:
25
08
2020
accepted:
29
10
2020
entrez:
2
12
2020
pubmed:
3
12
2020
medline:
28
9
2021
Statut:
epublish
Résumé
The ability to distinguish resident microglia from infiltrating myeloid cells by flow cytometry-based surface phenotyping is an important technique for examining age-related neuroinflammation. The most commonly used surface markers for the identification of microglia include CD45 (low-intermediate expression), CD11b, Tmem119, and P2RY12. In this study, we examined changes in expression levels of these putative microglia markers in in vivo animal models of stroke, cerebral amyloid angiopathy (CAA), and aging as well as in an ex vivo LPS-induced inflammation model. We demonstrate that Tmem119 and P2RY12 expression is evident within both CD45 We recommend future studies approach microglia identification by flow cytometry with caution, particularly in the absence of the use of a combination of markers validated for the specific neuroinflammation model of interest. The subpopulation of resident microglia residing within the "infiltrating myeloid" population, albeit small, may be functionally important in maintaining immune vigilance in the brain thus should not be overlooked in neuroimmunological studies.
Sections du résumé
BACKGROUND
BACKGROUND
The ability to distinguish resident microglia from infiltrating myeloid cells by flow cytometry-based surface phenotyping is an important technique for examining age-related neuroinflammation. The most commonly used surface markers for the identification of microglia include CD45 (low-intermediate expression), CD11b, Tmem119, and P2RY12.
METHODS
METHODS
In this study, we examined changes in expression levels of these putative microglia markers in in vivo animal models of stroke, cerebral amyloid angiopathy (CAA), and aging as well as in an ex vivo LPS-induced inflammation model.
RESULTS
RESULTS
We demonstrate that Tmem119 and P2RY12 expression is evident within both CD45
CONCLUSION
CONCLUSIONS
We recommend future studies approach microglia identification by flow cytometry with caution, particularly in the absence of the use of a combination of markers validated for the specific neuroinflammation model of interest. The subpopulation of resident microglia residing within the "infiltrating myeloid" population, albeit small, may be functionally important in maintaining immune vigilance in the brain thus should not be overlooked in neuroimmunological studies.
Identifiants
pubmed: 33261619
doi: 10.1186/s12974-020-02019-5
pii: 10.1186/s12974-020-02019-5
pmc: PMC7709276
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
366Subventions
Organisme : NINDS NIH HHS
ID : R01 NS094543
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS103592
Pays : United States
Organisme : NINDS NIH HHS
ID : R21-NS114768-01A1
Pays : United States
Organisme : NIA NIH HHS
ID : RF1 AG058463
Pays : United States
Organisme : NIA NIH HHS
ID : 1-RF1-AG058463-01
Pays : United States
Organisme : NINDS NIH HHS
ID : F31 NS118984
Pays : United States
Organisme : NCATS NIH HHS
ID : TL1 TR003169
Pays : United States
Organisme : NINDS NIH HHS
ID : 5-R01-NS103592-02
Pays : United States
Organisme : NINDS NIH HHS
ID : 5-R01-NS094543-04
Pays : United States
Organisme : NINDS NIH HHS
ID : 1F31NS118984-01
Pays : United States
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