Epistasis-driven identification of SLC25A51 as a regulator of human mitochondrial NAD import.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
01 12 2020
01 12 2020
Historique:
received:
03
10
2020
accepted:
02
11
2020
entrez:
2
12
2020
pubmed:
3
12
2020
medline:
15
12
2020
Statut:
epublish
Résumé
About a thousand genes in the human genome encode for membrane transporters. Among these, several solute carrier proteins (SLCs), representing the largest group of transporters, are still orphan and lack functional characterization. We reasoned that assessing genetic interactions among SLCs may be an efficient way to obtain functional information allowing their deorphanization. Here we describe a network of strong genetic interactions indicating a contribution to mitochondrial respiration and redox metabolism for SLC25A51/MCART1, an uncharacterized member of the SLC25 family of transporters. Through a combination of metabolomics, genomics and genetics approaches, we demonstrate a role for SLC25A51 as enabler of mitochondrial import of NAD, showcasing the potential of genetic interaction-driven functional gene deorphanization.
Identifiants
pubmed: 33262325
doi: 10.1038/s41467-020-19871-x
pii: 10.1038/s41467-020-19871-x
pmc: PMC7708531
doi:
Substances chimiques
UCP1 protein, human
0
Uncoupling Protein 1
0
NAD
0U46U6E8UK
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6145Commentaires et corrections
Type : CommentIn
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