Fluid Shear Stress Enhances the Phagocytic Response of Astrocytes.
astrocyte
laser ablation
laser nanosurgery
mechanotransduction
phagocytosis
shear stress
Journal
Frontiers in bioengineering and biotechnology
ISSN: 2296-4185
Titre abrégé: Front Bioeng Biotechnol
Pays: Switzerland
ID NLM: 101632513
Informations de publication
Date de publication:
2020
2020
Historique:
received:
19
08
2020
accepted:
15
10
2020
entrez:
2
12
2020
pubmed:
3
12
2020
medline:
3
12
2020
Statut:
epublish
Résumé
Astrocytes respond to brain injury at a cellular level by the process of reactive astrogliosis, and are able to adjust their response according to the severity of the insult. Included in the reactive response is the process of phagocytosis, where astrocytes clean up surrounding cellular debris from damaged cells. In this study, we observe the process of phagocytosis by primary cortical astrocytes in the presence of media flow across the apical surface of the cells. Both static and cells under flow conditions respond consistently via phagocytosis of laser-induced cellular debris. We found that astrocytes exposed to shear flow initiate phagocytosis at a consistently faster rate than cells observed under static conditions. Shear forces created by laminar flow were analyzed as well as the flow fields created around astrocyte cells. Results suggest astrocyte phagocytosis is a mechanosensitive response, thus revealing the potential to enhance astrocyte phagocytic cleanup of damaged nervous tissue.
Identifiants
pubmed: 33262978
doi: 10.3389/fbioe.2020.596577
pmc: PMC7686466
doi:
Banques de données
figshare
['10.6084/m9.figshare.12827795']
Types de publication
Journal Article
Langues
eng
Pagination
596577Informations de copyright
Copyright © 2020 Wakida, Cruz, Pouladian, Berns and Preece.
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