SCENITH: A Flow Cytometry-Based Method to Functionally Profile Energy Metabolism with Single-Cell Resolution.
cell culture media and metabolism
functional assay metabolism single cells
metabolic function by flow cytometry
metabolic gene signatures
metabolic profiling of blood samples
metabolism analysis in samples from patients
protein synthesis and metabolism
translation and metabolism
tumor immunometabolism
Journal
Cell metabolism
ISSN: 1932-7420
Titre abrégé: Cell Metab
Pays: United States
ID NLM: 101233170
Informations de publication
Date de publication:
01 12 2020
01 12 2020
Historique:
received:
18
03
2020
revised:
09
08
2020
accepted:
11
11
2020
entrez:
2
12
2020
pubmed:
3
12
2020
medline:
23
11
2021
Statut:
ppublish
Résumé
Energetic metabolism reprogramming is critical for cancer and immune responses. Current methods to functionally profile the global metabolic capacities and dependencies of cells are performed in bulk. We designed a simple method for complex metabolic profiling called SCENITH, for single-cell energetic metabolism by profiling translation inhibition. SCENITH allows for the study of metabolic responses in multiple cell types in parallel by flow cytometry. SCENITH is designed to perform metabolic studies ex vivo, particularly for rare cells in whole blood samples, avoiding metabolic biases introduced by culture media. We analyzed myeloid cells in solid tumors from patients and identified variable metabolic profiles, in ways that are not linked to their lineage or their activation phenotype. SCENITH's ability to reveal global metabolic functions and determine complex and linked immune-phenotypes in rare cell subpopulations will contribute to the information needed for evaluating therapeutic responses or patient stratification.
Identifiants
pubmed: 33264598
pii: S1550-4131(20)30602-1
doi: 10.1016/j.cmet.2020.11.007
pmc: PMC8407169
mid: NIHMS1734273
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1063-1075.e7Subventions
Organisme : NCI NIH HHS
ID : R01 CA197363
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA217864
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests. There are restrictions to the commercial use of SCENITH due to a pending patent application (PCT/EP2020/060486).
Références
Cell. 2015 Sep 10;162(6):1229-41
pubmed: 26321679
Biochem J. 1995 Nov 15;312 ( Pt 1):163-7
pubmed: 7492307
Blood. 2012 Aug 16;120(7):1422-31
pubmed: 22786879
Immunity. 2012 Jan 27;36(1):68-78
pubmed: 22206904
Cell Metab. 2020 Nov 3;32(5):710-725
pubmed: 33027638
Nat Methods. 2017 Jan;14(1):57-60
pubmed: 27842059
Nat Rev Immunol. 2019 May;19(5):324-335
pubmed: 30820043
Cell. 2019 Jan 10;176(1-2):73-84.e15
pubmed: 30612742
Nucleic Acids Res. 2000 Mar 1;28(5):1176-82
pubmed: 10666460
N Engl J Med. 2018 Dec 13;379(24):2342-2350
pubmed: 30280658
Genome Res. 2015 Dec;25(12):1860-72
pubmed: 26430063
Mol Biol Cell. 2007 Apr;18(4):1437-46
pubmed: 17301289
Blood. 2010 Jun 10;115(23):4742-9
pubmed: 20351312
J Cell Sci. 2018 May 31;131(10):
pubmed: 29700204
Nat Protoc. 2019 Feb;14(2):441-460
pubmed: 30610239
Cell. 2015 May 21;161(5):1202-1214
pubmed: 26000488
Science. 1993 Feb 26;259(5099):1264-5
pubmed: 8446896
Nat Rev Immunol. 2019 May;19(5):291-304
pubmed: 30679807
Science. 2006 Sep 29;313(5795):1960-4
pubmed: 17008531
Proc Natl Acad Sci U S A. 1967 Dec;58(6):2401-7
pubmed: 5242216
FEBS Lett. 1999 Nov 26;462(1-2):43-6
pubmed: 10580088
Exp Cell Res. 1973 Mar 15;77(1):127-35
pubmed: 4690164
Science. 2017 Apr 21;356(6335):
pubmed: 28428369
Commun Biol. 2020 Jun 12;3(1):305
pubmed: 32533056
N Engl J Med. 2017 Oct 5;377(14):1345-1356
pubmed: 28889792
Proc Natl Acad Sci U S A. 2013 Aug 27;110(35):14336-41
pubmed: 23940348
Nature. 2009 Jul 2;460(7251):103-7
pubmed: 19494812
Cell Metab. 2017 Nov 7;26(5):788-800.e6
pubmed: 28889950
Immunol Rev. 2017 Nov;280(1):102-111
pubmed: 29027220
Z Naturforsch B. 1958 Aug;13B(8):515-6
pubmed: 13593654
Curr Opin Genet Dev. 2018 Feb;48:104-111
pubmed: 29179096
Cell Metab. 2019 Jul 2;30(1):36-50
pubmed: 31269428
Biochem J. 1971 Feb;121(4):683-94
pubmed: 5114978
J Exp Med. 2015 Aug 24;212(9):1345-60
pubmed: 26261266
J Immunol. 2012 Sep 1;189(5):2151-8
pubmed: 22826320
Front Immunol. 2019 Apr 25;10:775
pubmed: 31073300
J Immunol. 2016 Aug 15;197(4):1498-506
pubmed: 27385780
Comput Struct Biotechnol J. 2020 Apr 24;18:1074-1083
pubmed: 32435426
Nat Protoc. 2012 May 10;7(6):1068-85
pubmed: 22576106
Nat Methods. 2009 Apr;6(4):275-7
pubmed: 19305406
Nat Biotechnol. 2021 Feb;39(2):186-197
pubmed: 32868913
Redox Biol. 2018 Jun;16:381-387
pubmed: 29627745
Nat Biotechnol. 2015 May;33(5):495-502
pubmed: 25867923