Targeting Neuropilin-1 with Nanobodies Reduces Colorectal Carcinoma Development.

cancer immune checkpoint immunotherapy nanobody neuropilin-1 plexin semaphorin single-domain antibody fragment tumor-associated macrophage

Journal

Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829

Informations de publication

Date de publication:
30 Nov 2020
Historique:
received: 22 10 2020
revised: 24 11 2020
accepted: 26 11 2020
entrez: 3 12 2020
pubmed: 4 12 2020
medline: 4 12 2020
Statut: epublish

Résumé

Neuropilin-1 (NRP-1) is a co-receptor for semaphorins and vascular endothelial growth factor (VEGF) family members that can be expressed on cancer cells and tumor-infiltrating myeloid, endothelial and lymphoid cells. It has been linked to a tumor-promoting environment upon interaction with semaphorin 3A (Sema3A). Nanobodies (Nbs) targeting NRP-1 were generated for their potential to hamper the NRP-1/Sema3A interaction and their impact on colorectal carcinoma (CRC) development was evaluated in vivo through the generation of anti-NRP-1-producing CRC cells. We observed that tumor growth was significantly delayed and survival prolonged when the anti-NRP-1 Nbs were produced in vivo. We further analyzed the tumor microenvironment and observed that the pro-inflammatory MHC-II

Identifiants

pubmed: 33266104
pii: cancers12123582
doi: 10.3390/cancers12123582
pmc: PMC7760077
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : MIUR-PRIN
ID : 2017TATYMP
Organisme : Kom op tegen Kanker
ID : ANI146
Organisme : Fonds Wetenschappelijk Onderzoek
ID : S000218N
Organisme : Fonds Wetenschappelijk Onderzoek
ID : 1S24817N
Organisme : Vrije Universiteit Brussel
ID : SRP48

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Auteurs

Yannick De De Vlaeminck (Y)

Laboratory for Molecular and Cellular Therapy, Department of Biomedical Sciences, Vrije Universiteit Brussel, 1090 Brussels, Belgium.

Stefano Bonelli (S)

Laboratory for Cellular and Molecular Immunology, Vrije Universiteit Brussel, 1040 Brussels, Belgium.
Myeloid Cell Immunology Lab, VIB Center for Inflammation Research, 1040 Brussels, Belgium.

Robin Maximilian Awad (RM)

Laboratory for Molecular and Cellular Therapy, Department of Biomedical Sciences, Vrije Universiteit Brussel, 1090 Brussels, Belgium.

Maarten Dewilde (M)

VIB Discovery Sciences, 3000 Leuven, Belgium.

Sabrina Rizzolio (S)

Candiolo Cancer Institute-FPO, IRCCS, 10060 Candiolo, Italy.

Quentin Lecocq (Q)

Laboratory for Molecular and Cellular Therapy, Department of Biomedical Sciences, Vrije Universiteit Brussel, 1090 Brussels, Belgium.

Evangelia Bolli (E)

Laboratory for Cellular and Molecular Immunology, Vrije Universiteit Brussel, 1040 Brussels, Belgium.
Myeloid Cell Immunology Lab, VIB Center for Inflammation Research, 1040 Brussels, Belgium.

Ana Rita Santos (AR)

VIB Discovery Sciences, 3000 Leuven, Belgium.

Damya Laoui (D)

Laboratory for Cellular and Molecular Immunology, Vrije Universiteit Brussel, 1040 Brussels, Belgium.
Myeloid Cell Immunology Lab, VIB Center for Inflammation Research, 1040 Brussels, Belgium.

Steve Schoonooghe (S)

Laboratory for Cellular and Molecular Immunology, Vrije Universiteit Brussel, 1040 Brussels, Belgium.
Myeloid Cell Immunology Lab, VIB Center for Inflammation Research, 1040 Brussels, Belgium.

Luca Tamagnone (L)

Department of Life Sciences and Public Health, Università Cattolica del Sacro Cuore, 00100 Rome, Italy.
Department of Oncology, Fondazione Policlinico Universitario "A. Gemelli", IRCCS, 00100 Rome, Italy.

Cleo Goyvaerts (C)

Laboratory for Molecular and Cellular Therapy, Department of Biomedical Sciences, Vrije Universiteit Brussel, 1090 Brussels, Belgium.

Massimiliano Mazzone (M)

Laboratory of Tumor Inflammation and Angiogenesis, VIB Center for Cancer Biology, 3000 Leuven, Belgium.
Department of Oncology, Laboratory of Tumor Inflammation and Angiogenesis, Center for Cancer Biology, KU Leuven, 3000 Leuven, Belgium.

Karine Breckpot (K)

Laboratory for Molecular and Cellular Therapy, Department of Biomedical Sciences, Vrije Universiteit Brussel, 1090 Brussels, Belgium.

Jo A Van Ginderachter (JA)

Laboratory for Cellular and Molecular Immunology, Vrije Universiteit Brussel, 1040 Brussels, Belgium.
Myeloid Cell Immunology Lab, VIB Center for Inflammation Research, 1040 Brussels, Belgium.

Classifications MeSH