High-Sensitivity Assessment of Phagocytosis by Persistent Association-Based Normalization.
Journal
Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
Titre abrégé: J Immunol
Pays: United States
ID NLM: 2985117R
Informations de publication
Date de publication:
01 01 2021
01 01 2021
Historique:
received:
10
01
2020
accepted:
29
10
2020
pubmed:
4
12
2020
medline:
21
5
2021
entrez:
3
12
2020
Statut:
ppublish
Résumé
Phagocytosis is measured as a functional outcome in many research fields, but accurate quantification can be challenging, with no robust method available for cross-laboratory reproducibility. In this study, we identified a simple, measurable parameter, persistent prey-phagocyte association, to use for normalization and dose-response analysis. We apply this in a straightforward analytical method, persistent association-based normalization, in which the multiplicity of prey (MOP) ratio needed to elicit half of the phagocytes to associate persistently (MOP
Identifiants
pubmed: 33268484
pii: jimmunol.2000032
doi: 10.4049/jimmunol.2000032
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
214-224Informations de copyright
Copyright © 2020 by The American Association of Immunologists, Inc.