Leishmanicidal, cytotoxic and apoptotic effects of
Apoptosis
Gossypium hirsutum
chromatography
cutaneous leishmaniasis
cytotoxicity
leishmanicidal activity
Journal
Journal of vector borne diseases
ISSN: 0972-9062
Titre abrégé: J Vector Borne Dis
Pays: India
ID NLM: 101212761
Informations de publication
Date de publication:
Historique:
entrez:
3
12
2020
pubmed:
1
10
2019
medline:
5
8
2021
Statut:
ppublish
Résumé
Leishmaniasis is a major global health problem with no safe and effective therapeutic drugs. This study evaluated the cytotoxic and apoptotic effects of crude extract and fractions of Gossypium hirsutum bulb on Leishmania major stages using advanced experimental models. Bulbs of G. hirsutum were collected from the Kerman province of Iran. The bulb was extracted using Soxhlet apparatus and different fractions were obtained by column chromatography (CC). Different concentrations of the extract and the fractions were evaluated against L. major and compared with Glucantime®. The cytotoxicity and apoptotic values were analysed by flow cytometry. The fractions obtained in CC were monitored by thin layer chromatography, and fractions with similar chromatographic patterns were mixed. The extract and two fractions, F4 and F5 inhibited the proliferation of L. major promastigotes and amastigotes in a dose-dependent manner at 72 h post-treatment. No significant cytotoxic effects were observed for extract and fractions, as the selectivity index was over 1000, far beyond >10. The mean apoptotic values for L. major were superior to those of Glucantime Both the crude extract and fractions (F4 and F5) had significant antileishmanial effects on L. major stages, and were were superior relative to Glucantime
Sections du résumé
BACKGROUND & OBJECTIVES
OBJECTIVE
Leishmaniasis is a major global health problem with no safe and effective therapeutic drugs. This study evaluated the cytotoxic and apoptotic effects of crude extract and fractions of Gossypium hirsutum bulb on Leishmania major stages using advanced experimental models.
METHODS
METHODS
Bulbs of G. hirsutum were collected from the Kerman province of Iran. The bulb was extracted using Soxhlet apparatus and different fractions were obtained by column chromatography (CC). Different concentrations of the extract and the fractions were evaluated against L. major and compared with Glucantime®. The cytotoxicity and apoptotic values were analysed by flow cytometry. The fractions obtained in CC were monitored by thin layer chromatography, and fractions with similar chromatographic patterns were mixed.
RESULTS
RESULTS
The extract and two fractions, F4 and F5 inhibited the proliferation of L. major promastigotes and amastigotes in a dose-dependent manner at 72 h post-treatment. No significant cytotoxic effects were observed for extract and fractions, as the selectivity index was over 1000, far beyond >10. The mean apoptotic values for L. major were superior to those of Glucantime
INTERPRETATION & CONCLUSION
CONCLUSIONS
Both the crude extract and fractions (F4 and F5) had significant antileishmanial effects on L. major stages, and were were superior relative to Glucantime
Identifiants
pubmed: 33269733
pii: JVectorBorneDis_2019_56_4_330_302036
doi: 10.4103/0972-9062.302036
doi:
Substances chimiques
Antiprotozoal Agents
0
Plant Extracts
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
330-338Déclaration de conflit d'intérêts
None