Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency.
chimaera
epiblast
formative pluripotency
lineage induction
pluripotent stem cell
primordial germ cell
self-renewal
Journal
Cell stem cell
ISSN: 1875-9777
Titre abrégé: Cell Stem Cell
Pays: United States
ID NLM: 101311472
Informations de publication
Date de publication:
04 03 2021
04 03 2021
Historique:
received:
06
09
2019
revised:
03
09
2020
accepted:
09
11
2020
pubmed:
4
12
2020
medline:
20
5
2021
entrez:
3
12
2020
Statut:
ppublish
Résumé
Pluripotent cells emerge as a naive founder population in the blastocyst, acquire capacity for germline and soma formation, and then undergo lineage priming. Mouse embryonic stem cells (ESCs) and epiblast-derived stem cells (EpiSCs) represent the initial naive and final primed phases of pluripotency, respectively. Here, we investigate the intermediate formative stage. Using minimal exposure to specification cues, we derive stem cells from formative mouse epiblast. Unlike ESCs or EpiSCs, formative stem (FS) cells respond directly to germ cell induction. They colonize somatic tissues and germline in chimeras. Whole-transcriptome analyses show similarity to pre-gastrulation formative epiblast. Signal responsiveness and chromatin accessibility features reflect lineage capacitation. Furthermore, FS cells show distinct transcription factor dependencies, relying critically on Otx2. Finally, FS cell culture conditions applied to human naive cells or embryos support expansion of similar stem cells, consistent with a conserved staging post on the trajectory of mammalian pluripotency.
Identifiants
pubmed: 33271069
pii: S1934-5909(20)30543-9
doi: 10.1016/j.stem.2020.11.005
pmc: PMC7939546
mid: EMS119154
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
453-471.e8Subventions
Organisme : Medical Research Council
ID : MC_PC_17230
Pays : United Kingdom
Organisme : Medical Research Council
ID : G1100526
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P010423/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P00072X/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P00072X/2
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 203151
Pays : United Kingdom
Commentaires et corrections
Type : CommentIn
Type : ErratumIn
Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests
Références
Nat Protoc. 2014 Jan;9(1):171-81
pubmed: 24385147
Development. 2015 Sep 15;142(18):3090-9
pubmed: 26395138
Cell. 2014 Sep 11;158(6):1254-1269
pubmed: 25215486
Nat Biotechnol. 2009 Mar;27(3):275-80
pubmed: 19252484
Development. 2017 Aug 1;144(15):2748-2763
pubmed: 28765214
Development. 2019 Apr 3;146(7):
pubmed: 30944104
Nature. 2007 Jul 12;448(7150):191-5
pubmed: 17597762
Cell Stem Cell. 2019 May 2;24(5):785-801.e7
pubmed: 31031137
Genes Dev. 1999 Jul 15;13(14):1834-46
pubmed: 10421635
Science. 2004 Jan 30;303(5658):666-9
pubmed: 14752160
Nat Cell Biol. 2020 May;22(5):534-545
pubmed: 32367046
Nucleic Acids Res. 2016 Jul 8;44(W1):W160-5
pubmed: 27079975
Science. 2014 Jun 6;344(6188):1156-1160
pubmed: 24904165
Development. 1991 Nov;113(3):891-911
pubmed: 1821858
Nature. 2016 Sep 1;537(7618):57-62
pubmed: 27556940
Development. 2007 Aug;134(16):2889-94
pubmed: 17660197
Biochem Soc Trans. 2003 Feb;31(Pt 1):45-9
pubmed: 12546651
Nat Protoc. 2009;4(1):44-57
pubmed: 19131956
Cell Rep. 2014 Jun 26;7(6):1968-81
pubmed: 24931607
Cell. 2015 Jan 15;160(1-2):253-68
pubmed: 25543152
Cell Stem Cell. 2009 Jun 5;4(6):487-92
pubmed: 19497275
Bioinformatics. 2014 Apr 1;30(7):1008-9
pubmed: 24363377
Cell Stem Cell. 2016 Nov 3;19(5):587-592
pubmed: 27814480
Nat Protoc. 2016 Oct;11(10):1833-50
pubmed: 27583644
Cell. 2009 May 1;137(3):571-84
pubmed: 19410550
PLoS One. 2012;7(9):e45220
pubmed: 23028858
Development. 2012 Jul;139(13):2288-98
pubmed: 22669820
Stem Cell Reports. 2014 May 22;2(6):881-95
pubmed: 24936473
Philos Trans R Soc Lond B Biol Sci. 2014 Dec 5;369(1657):
pubmed: 25349449
Genome Biol. 2014;15(12):550
pubmed: 25516281
Genome Biol. 2014;15(12):545
pubmed: 25476147
Development. 1996 Jan;122(1):243-52
pubmed: 8565836
Cell Stem Cell. 2014 Feb 6;14(2):237-52
pubmed: 24412311
Cell. 2011 Aug 19;146(4):519-32
pubmed: 21820164
J Cell Sci. 2007 Jun 15;120(Pt 12):2078-90
pubmed: 17535850
Dev Cell. 2015 Nov 9;35(3):366-82
pubmed: 26555056
Nat Cell Biol. 2006 May;8(5):532-8
pubmed: 16570078
Stem Cell Reports. 2016 Apr 12;6(4):437-446
pubmed: 26947977
Cell Stem Cell. 2011 Mar 4;8(3):318-25
pubmed: 21362571
Dev Cell. 2009 Apr;16(4):607-13
pubmed: 19386269
Genome Biol. 2019 Dec 23;20(1):296
pubmed: 31870423
Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15656-60
pubmed: 15505202
Development. 1994 Jul;120(7):1919-28
pubmed: 7924997
Nature. 2013 Sep 12;501(7466):222-6
pubmed: 23913270
Genes Dev. 1995 Sep 1;9(17):2105-16
pubmed: 7657163
Sci Rep. 2019 Mar 6;9(1):3637
pubmed: 30842444
Nature. 2007 Jul 12;448(7150):196-9
pubmed: 17597760
Philos Trans R Soc Lond B Biol Sci. 1985 Dec 17;312(1153):163-78
pubmed: 2869527
Cell Stem Cell. 2016 Oct 6;19(4):502-515
pubmed: 27424783
PLoS One. 2009 Nov 05;4(11):e7708
pubmed: 19890402
Nat Commun. 2020 May 15;11(1):2420
pubmed: 32415101
Nat Genet. 2009 Dec;41(12):1295-302
pubmed: 19898483
Cell. 2006 Apr 21;125(2):315-26
pubmed: 16630819
Proc Natl Acad Sci U S A. 1997 May 27;94(11):5709-12
pubmed: 9159137
Development. 2007 Aug;134(16):2895-902
pubmed: 17660198
Nat Methods. 2011 May;8(5):424-9
pubmed: 21478862
Stem Cell Reports. 2015 Jan 13;4(1):114-128
pubmed: 25544567
Trends Genet. 1998 Jul;14(7):277-84
pubmed: 9676530
Annu Rev Cell Dev Biol. 2015;31:429-51
pubmed: 26393776
Nat Biotechnol. 2018 Jun;36(5):411-420
pubmed: 29608179
Cell Stem Cell. 2017 Jan 5;20(1):18-28
pubmed: 28061351
Development. 2006 Jul;133(13):2497-505
pubmed: 16728477
Nucleic Acids Res. 2019 May 7;47(8):e47
pubmed: 30783653
Development. 2019 Apr 5;146(7):
pubmed: 30890572
Nat Methods. 2013 Dec;10(12):1213-8
pubmed: 24097267
Dev Cell. 2016 Mar 21;36(6):681-97
pubmed: 27003939
Nature. 1988 Dec 15;336(6200):684-7
pubmed: 3143916
Methods Mol Biol. 2006;329:91-8
pubmed: 16845986
Development. 2013 Jan 1;140(1):43-55
pubmed: 23154415
Genome Biol. 2008;9(9):R137
pubmed: 18798982
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
Development. 1997 Mar;124(5):1033-44
pubmed: 9056778
Cell Stem Cell. 2014 Jan 2;14(1):107-20
pubmed: 24139757
Nat Biotechnol. 2012 Feb 26;30(3):278-82
pubmed: 22371082
Cell Rep. 2019 Mar 5;26(10):2593-2607.e3
pubmed: 30840884
Development. 2010 Mar;137(6):881-90
pubmed: 20179094
Mol Cell. 2010 May 28;38(4):576-89
pubmed: 20513432
Cell Stem Cell. 2014 Jun 5;14(6):838-53
pubmed: 24905168
Cell Stem Cell. 2019 Jul 3;25(1):120-136.e10
pubmed: 31155483
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Int J Dev Biol. 1997 Apr;41(2):235-43
pubmed: 9184330
Semin Cell Dev Biol. 2014 Aug;32:73-9
pubmed: 24704361
PLoS One. 2013 Dec 06;8(12):e81148
pubmed: 24324667
Development. 2019 Mar 26;146(6):
pubmed: 30914406
Nature. 2019 Aug;572(7770):528-532
pubmed: 31391582
Nature. 2020 Jan;577(7791):537-542
pubmed: 31830756
Cell Stem Cell. 2014 Oct 2;15(4):416-430
pubmed: 25280218
Development. 2017 Feb 1;144(3):365-373
pubmed: 28143843
Nature. 1987 Mar 19-25;326(6110):292-5
pubmed: 3821905
Stem Cell Reports. 2017 Jul 11;9(1):77-91
pubmed: 28669603
Elife. 2015 Nov 09;4:
pubmed: 26551560
Dev Growth Differ. 2018 Jan;60(1):44-52
pubmed: 29359419
EMBO J. 2019 Jun 17;38(12):
pubmed: 31036553
J Embryol Exp Morphol. 1985 Aug;88:349-63
pubmed: 4078538
Proc Natl Acad Sci U S A. 1975 Dec;72(12):5099-102
pubmed: 1108013
Nature. 1988 Dec 15;336(6200):688-90
pubmed: 3143917
Cell Stem Cell. 2018 Jun 01;22(6):919-928.e6
pubmed: 29804891
Development. 2009 Apr;136(7):1063-9
pubmed: 19224983
Development. 1992 Mar;114(3):755-68
pubmed: 1618140
Nat Biotechnol. 2007 Jun;25(6):681-6
pubmed: 17529971
Genome Biol. 2013 Apr 25;14(4):R36
pubmed: 23618408
Development. 2014 Mar;141(6):1209-21
pubmed: 24595287
Stem Cell Reports. 2017 Jan 10;8(1):1-10
pubmed: 28017658
Nat Genet. 1999 Aug;22(4):361-5
pubmed: 10431240
Bioinformatics. 2011 Jun 1;27(11):1571-2
pubmed: 21493656
PLoS One. 2015 Apr 14;10(4):e0123467
pubmed: 25875838
Stem Cell Reports. 2019 Dec 10;13(6):1083-1098
pubmed: 31708477
Nature. 2005 Jul 14;436(7048):207-13
pubmed: 15937476
Stem Cell Reports. 2018 Jun 5;10(6):1895-1907
pubmed: 29779895
Dev Biol. 1995 Apr;168(2):342-57
pubmed: 7729574
Nature. 2001 Jun 21;411(6840):965-9
pubmed: 11418863
Development. 1991 Jun;112(2):397-406
pubmed: 1794310
Cell Rep. 2016 Jun 21;15(12):2651-64
pubmed: 27292645
J Cell Sci. 1999 Mar;112 ( Pt 5):601-12
pubmed: 9973595
Nature. 2016 Jan 21;529(7586):403-407
pubmed: 26751055
Cell. 2019 May 2;177(4):910-924.e22
pubmed: 30982595
Development. 2015 Jan 1;142(1):9-12
pubmed: 25516964
Development. 2017 Apr 1;144(7):1221-1234
pubmed: 28174249
Cell Stem Cell. 2015 Aug 6;17(2):178-94
pubmed: 26189426
Cell. 2010 Nov 12;143(4):617-27
pubmed: 21056461
Nat Cell Biol. 2014 Jun;16(6):516-28
pubmed: 24859004